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<p><i>Withania somnifera</i> (L.) Dunal (family Solanaceae), an important plant with therapeutic potential, is included in traditional Ayurvedic medications. The presence of withanolides (secondary metabolites, naturally occurring steroids), including withaferin-A, withanolide-A, withanone, and withanolide-D, are primarily responsible for the pharmacological properties of this plant. Withaferin-A and withanolide-A have garnered significant attention among them because of their anticancer activities against a number of cancer cell lines as well as neuro-protective, immunomodulatory, nephroprotective, cardiovascular, and anti-diabetic activities. The establishment of aseptic cultures for clonal mass multiplication of <i>W. somnifera</i> along with withaferin-A and withanolide-A production were the objective of the current study. Three cytokinins namely 6-benzyladenine (BA), kinetin (Kn), and <i>meta</i>-Topolin (<i>m</i>T) were used in varying concentrations (0.5–3 mg/L). Maximum shoot proliferation (8.3 shoots/explant) was achieved by using (2 mg/L) <i>m</i>T, a novel cytokinin. Principal component analysis, correlation matrix, clustering, and network plot analyses demonstrated the relationship between plant growth regulators according to how they affected the in vitro growth characteristics that were being examined. Among two distinct auxins such as indole-3-acetic acid and indole-3-butyric acid (IBA), the treatment 2&#xa0;mg/L IBA was proved to be optimal for inducing the earliest (9 days), maximum number (38), and most elongated (37.7 mm) root growth. The micropropagated plantlets were transferred to the sand for their acclimatization, and 95% survival was recorded. The genetic uniformity of in vitro-regenerated plantlets was verified first through chromosomal study, flow cytometry, and then with start codon targeted polymorphism markers. The presence of withaferin-A in leaf extract and withanolide-A in root extract were quantified by high-performance liquid chromatography. The control (mother) plant produced 1.2 mg/g dry weight (DW) withaferin-A and 2.1 mg/g DW withanolide-A, while the micropropagated plantlet produced 1.7 mg/g DW withaferin-A and 2.35 mg/g DW withanolide-A, respectively.</p>

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In vitro multiplication, flowering, and production of withaferin-A and withanolide-A from meta-topolin-induced genetically stable Withania somnifera (L.) Dunal plantlets

  • 期刊论文

  • 发表日期：

    2025年5月16日

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  • Sandipan Bandyopadhyay,
  • Tsama Subrahmanyeswari,
  • Jayoti Majumder Sarkar,
  • Suchita N. Kamble,
  • Sudhir Singh,
  • Sanjay Kumar Singh,
  • Somnath Bhattacharyya,
  • Saikat Gantait

摘要

Withania somnifera (L.) Dunal (family Solanaceae), an important plant with therapeutic potential, is included in traditional Ayurvedic medications. The presence of withanolides (secondary metabolites, naturally occurring steroids), including withaferin-A, withanolide-A, withanone, and withanolide-D, are primarily responsible for the pharmacological properties of this plant. Withaferin-A and withanolide-A have garnered significant attention among them because of their anticancer activities against a number of cancer cell lines as well as neuro-protective, immunomodulatory, nephroprotective, cardiovascular, and anti-diabetic activities. The establishment of aseptic cultures for clonal mass multiplication of W. somnifera along with withaferin-A and withanolide-A production were the objective of the current study. Three cytokinins namely 6-benzyladenine (BA), kinetin (Kn), and meta-Topolin (mT) were used in varying concentrations (0.5–3 mg/L). Maximum shoot proliferation (8.3 shoots/explant) was achieved by using (2 mg/L) mT, a novel cytokinin. Principal component analysis, correlation matrix, clustering, and network plot analyses demonstrated the relationship between plant growth regulators according to how they affected the in vitro growth characteristics that were being examined. Among two distinct auxins such as indole-3-acetic acid and indole-3-butyric acid (IBA), the treatment 2 mg/L IBA was proved to be optimal for inducing the earliest (9 days), maximum number (38), and most elongated (37.7 mm) root growth. The micropropagated plantlets were transferred to the sand for their acclimatization, and 95% survival was recorded. The genetic uniformity of in vitro-regenerated plantlets was verified first through chromosomal study, flow cytometry, and then with start codon targeted polymorphism markers. The presence of withaferin-A in leaf extract and withanolide-A in root extract were quantified by high-performance liquid chromatography. The control (mother) plant produced 1.2 mg/g dry weight (DW) withaferin-A and 2.1 mg/g DW withanolide-A, while the micropropagated plantlet produced 1.7 mg/g DW withaferin-A and 2.35 mg/g DW withanolide-A, respectively.