Scholarlink

<p><i>Datura</i>, known for its rich pharmacological profile, presents significant potential in producing bioactive compounds. This study investigates the optimization of callus induction using plant growth regulators (PGRs) in <i>Datura innoxia</i> and <i>Datura stramonium</i> and secondary metabolites (SMs) production from calli treated with aluminium oxide (Al₂O₃) and tungsten oxide (WO₃) nanoparticles. For callus induction, leaf explants were inoculated on Murashige and Skoog (MS) medium supplemented with different concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D) and Thidiazuron (TDZ). Inducted calli were treated with varying concentrations of Al₂O₃ and WO₃ nanoparticles. Data analysis indicated that both PGRs significantly contribute to callus induction in both species. In the case of <i>Datura innoxia</i>, the maximum percentage (100%) of callus was achieved in 2,4-D 0.5, 1.0, and 1.5 mg/L supplemented media 1.5 mg/L TDZ supplemented media. The combinations, 1.0 + 0.5 mg/L, 1.5 + 0.5 mg/L, 0.5 + 1.5 mg/L and 1.0 + 1.5 mg/L 2,4-D and TDZ respectively provided the same callusing percentage. On the other hand, <i>c</i> showed maximum callus induction (100%) in the media supplemented with only 1.0 and 1.5 mg/L 2, 4-D. Phytochemical analysis of the nanoparticles treated calli in both species demonstrated the enhanced production of key alkaloids, phenolic compounds, and flavonoids compared to the non-treated calli. In terms of alkaloid production, both species showed height production (increment 263% and 283% respectively compared to the controls) with a supplantation of 60 mg/l WO₃. Around 80.8% increment of phenolic compounds was observed in <i>Datura stramonium</i> with 60 mg/g WO₃ supplementation. Again, 81.81% higher flavonoids production was observed in <i>D. innoxia</i> in response to WO₃. These findings suggest that Al₂O₃ and WO₃ nanoparticles can effectively enhance SMs production in <i>Datura</i> spp., offering a novel approach for maximizing the yield of valuable bioactive compounds. This research provides a foundation for the large-scale production of pharmaceutically important compounds from <i>Datura</i> through biotechnological interventions using nanoparticle technology.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Nanoparticle-mediated enhancement of alkaloid, phenolic, and flavonoid production in Datura callus cultures

  • 期刊论文

  • 发表日期：

    2025年5月26日

查看全文
  • Baan Munim Twaij,
  • Hashim K. Mohammed Al-oubaidi,
  • Md. Nazmul Hasan

摘要

Datura, known for its rich pharmacological profile, presents significant potential in producing bioactive compounds. This study investigates the optimization of callus induction using plant growth regulators (PGRs) in Datura innoxia and Datura stramonium and secondary metabolites (SMs) production from calli treated with aluminium oxide (Al₂O₃) and tungsten oxide (WO₃) nanoparticles. For callus induction, leaf explants were inoculated on Murashige and Skoog (MS) medium supplemented with different concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D) and Thidiazuron (TDZ). Inducted calli were treated with varying concentrations of Al₂O₃ and WO₃ nanoparticles. Data analysis indicated that both PGRs significantly contribute to callus induction in both species. In the case of Datura innoxia, the maximum percentage (100%) of callus was achieved in 2,4-D 0.5, 1.0, and 1.5 mg/L supplemented media 1.5 mg/L TDZ supplemented media. The combinations, 1.0 + 0.5 mg/L, 1.5 + 0.5 mg/L, 0.5 + 1.5 mg/L and 1.0 + 1.5 mg/L 2,4-D and TDZ respectively provided the same callusing percentage. On the other hand, c showed maximum callus induction (100%) in the media supplemented with only 1.0 and 1.5 mg/L 2, 4-D. Phytochemical analysis of the nanoparticles treated calli in both species demonstrated the enhanced production of key alkaloids, phenolic compounds, and flavonoids compared to the non-treated calli. In terms of alkaloid production, both species showed height production (increment 263% and 283% respectively compared to the controls) with a supplantation of 60 mg/l WO₃. Around 80.8% increment of phenolic compounds was observed in Datura stramonium with 60 mg/g WO₃ supplementation. Again, 81.81% higher flavonoids production was observed in D. innoxia in response to WO₃. These findings suggest that Al₂O₃ and WO₃ nanoparticles can effectively enhance SMs production in Datura spp., offering a novel approach for maximizing the yield of valuable bioactive compounds. This research provides a foundation for the large-scale production of pharmaceutically important compounds from Datura through biotechnological interventions using nanoparticle technology.