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<p>Carbohydrate sources play a critical role in supporting plant growth under in vitro conditions. <i>Centella asiatica</i> (L.) Urb., a highly valued medicinal plant, is widely recognized for its therapeutic properties, including neuroprotective, anti-inflammatory, and anti-neurodegenerative effects. Among various carbon sources, sucrose at a 3% concentration is most commonly utilized due to its efficient uptake, metabolism, and stability. In this study, treatment with 7% xylose resulted in the highest accumulation of chlorophyll <i>a</i> (0.86 g/100 g fresh weight and 0.41 g/100 g fresh weight) and chlorophyll <i>b</i> (0.41 g/100 g fresh weight). Furthermore, bioactive compound analyses revealed that 7% xylose significantly enhanced the levels of asiaticoside (33.51 ± 0.24 mg/g DW), madecassoside (43.02 ± 0.15 mg/g DW), asiatic acid (20.03 ± 0.07 mg/g DW), and madecassic acid (22.84 ± 0.14 mg/g DW). Additionally, the total phenolic content was maximized in treatments with 7% xylose, 5% xylose, and 7% galactose. Other monosaccharides, such as 5% fructose and 5% glucose, also promoted the accumulation of bioactive compounds. Among disaccharides, 1.5% sucrose increased madecassoside levels, while 7% maltose and 5% galactose elevated both asiaticoside and madecassoside concentrations. These results emphasize the significant impact of specific carbohydrate sources on chlorophyll biosynthesis and bioactive compound production in <i>C. asiatica</i>, highlighting their potential to optimize in vitro culture protocols for enhanced phytochemical yields.</p>

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Carbohydrates acting as enhancers of secondary metabolites synthesis in in vitro cultures of Centella asiatica (L.) Urb.

  • 期刊论文

  • 发表日期：

    2025年4月22日

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  • Suproteem Mukherjee,
  • Diptesh Biswas,
  • Biswajit Ghosh

摘要

Carbohydrate sources play a critical role in supporting plant growth under in vitro conditions. Centella asiatica (L.) Urb., a highly valued medicinal plant, is widely recognized for its therapeutic properties, including neuroprotective, anti-inflammatory, and anti-neurodegenerative effects. Among various carbon sources, sucrose at a 3% concentration is most commonly utilized due to its efficient uptake, metabolism, and stability. In this study, treatment with 7% xylose resulted in the highest accumulation of chlorophyll a (0.86 g/100 g fresh weight and 0.41 g/100 g fresh weight) and chlorophyll b (0.41 g/100 g fresh weight). Furthermore, bioactive compound analyses revealed that 7% xylose significantly enhanced the levels of asiaticoside (33.51 ± 0.24 mg/g DW), madecassoside (43.02 ± 0.15 mg/g DW), asiatic acid (20.03 ± 0.07 mg/g DW), and madecassic acid (22.84 ± 0.14 mg/g DW). Additionally, the total phenolic content was maximized in treatments with 7% xylose, 5% xylose, and 7% galactose. Other monosaccharides, such as 5% fructose and 5% glucose, also promoted the accumulation of bioactive compounds. Among disaccharides, 1.5% sucrose increased madecassoside levels, while 7% maltose and 5% galactose elevated both asiaticoside and madecassoside concentrations. These results emphasize the significant impact of specific carbohydrate sources on chlorophyll biosynthesis and bioactive compound production in C. asiatica, highlighting their potential to optimize in vitro culture protocols for enhanced phytochemical yields.