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<p><i>Echinacea purpurea</i>, a key medicinal herb of the Asteraceae family, is rich in antioxidants and anti-inflammatory compounds, making it valuable in pharmaceuticals. In vitro cultures enhance sustainable metabolite production, often stimulated by elicitors. This study employed TDZ to induce <i>E. purpurea</i> callus cultures and assessed the impact of melatonin on biomass, antioxidant potential, and secondary metabolites. The callus cultures treated with 25 µM melatonin showed the highest biomass (FW: 208.62&#xa0;g/L, DW: 11.87&#xa0;g/L), total phenolic (TPP: 132.8&#xa0;mg GAE/L), and flavonoid (TFP: 585.9&#xa0;mg QE/L) production. Antioxidant activity peaked at this concentration, with 92.7% DPPH scavenging and 63.2&#xa0;µg AAE/mg total antioxidant capacity. Moreover, the callus cultures exhibited notable enzyme inhibitory effects, with 68% inhibition against pancreatic lipase, 22% inhibition against alpha-glucosidase, and 40% inhibition against alpha-amylase, following treatment with 50 µM, 25 µM, and 10 µM of melatonin, respectively. These findings further highlight that the current approach should be further investigated to explore innovative treatments for obesity and diabetes. UPLC-MS analysis confirmed enhanced metabolite synthesis at 25–50 µM melatonin, while amino acid production peaked at 1µM. These findings highlight melatonin elicitation as an effective strategy for improving biomass, phenolics, and bioactivity in <i>E. purpurea</i> callus cultures.&#xa0;</p>

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Exploring melatonin’s influence on secondary metabolism of Echinacea purpurea callus cultures

  • 期刊论文

  • 发表日期：

    2025年4月24日

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  • Tehreem Mahmood,
  • Muhammad Uzair Javed,
  • Gouhar Zaman,
  • Bushra Khan,
  • Negin Zarei,
  • Cécile Abdallah,
  • Christophe Hano,
  • Johan-Owen De Craene,
  • Arnaud Lanoue,
  • Bilal Haider Abbasi,
  • Nathalie Giglioli-Guivarc’h

摘要

Echinacea purpurea, a key medicinal herb of the Asteraceae family, is rich in antioxidants and anti-inflammatory compounds, making it valuable in pharmaceuticals. In vitro cultures enhance sustainable metabolite production, often stimulated by elicitors. This study employed TDZ to induce E. purpurea callus cultures and assessed the impact of melatonin on biomass, antioxidant potential, and secondary metabolites. The callus cultures treated with 25 µM melatonin showed the highest biomass (FW: 208.62 g/L, DW: 11.87 g/L), total phenolic (TPP: 132.8 mg GAE/L), and flavonoid (TFP: 585.9 mg QE/L) production. Antioxidant activity peaked at this concentration, with 92.7% DPPH scavenging and 63.2 µg AAE/mg total antioxidant capacity. Moreover, the callus cultures exhibited notable enzyme inhibitory effects, with 68% inhibition against pancreatic lipase, 22% inhibition against alpha-glucosidase, and 40% inhibition against alpha-amylase, following treatment with 50 µM, 25 µM, and 10 µM of melatonin, respectively. These findings further highlight that the current approach should be further investigated to explore innovative treatments for obesity and diabetes. UPLC-MS analysis confirmed enhanced metabolite synthesis at 25–50 µM melatonin, while amino acid production peaked at 1µM. These findings highlight melatonin elicitation as an effective strategy for improving biomass, phenolics, and bioactivity in E. purpurea callus cultures.