Scholarlink

<p><i>Artemisia argyi</i> is a renowned Chinese medicinal herb extensively utilized for treating gynecological diseases. However, the application of bioreactor systems for the production of bioactive metabolites in <i>A. argyi</i> has yet to be explored. In this study, we established the callus cell suspension culture and genetic transformation systems for <i>A. argyi</i> for the first time. The optimal conditions for the culture system were determined to be an initial inoculation amount of 10%, a medium hormone ratio of 2.0&#xa0;mg/L 2,4-D + 0.5&#xa0;mg/L 6-BA + 1.0&#xa0;mg/L NAA, a medium volume of 25 mL, a shaking speed of 140&#xa0;rpm, and a light/dark cycle of 16&#xa0;h/8 hours. Under these cultivation conditions, the cell fresh weight reached 146.98&#xa0;g/L around the 20th day. Especially, the content of isochlorogenic acid A could reach to 321.06&#xa0;mg/L, which is 258.92 times higher than that in vivo. Furthermore, we developed an efficient genetic transformation system for <i>A. argyi</i> by <i>Agrobacterium tumefaciens</i> strain EHA105, achieving a maximum transformation efficiency of 60% under conditions that included pre-culturing for 4 days, a bacterial concentration (OD600) of 1.2, an inoculation time of 15&#xa0;min, and a co-cultivation period of 2 days. Using this system to overexpress <i>RUBY</i> gene, the content of betacyanin and betaxanthin in <i>A. argyi</i> callus could reach 535.79&#xa0;mg/L and 640.90&#xa0;mg/L, respectively. Based on the establishment of these two systems, we have devised a comprehensive and effective production strategy for active ingredients, which can be applied to the industrial production of bioactive compounds of <i>A. argyi</i> in the future.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Establishment of cell suspension culture and genetic transformation systems for production of bioactive metabolites of medicinal plant Artemisia argyi Lévl. et Vant

  • 期刊论文

  • 发表日期：

    2025年4月1日

查看全文
  • Yukun Li,
  • Sainan Peng,
  • Jingyi Zhang,
  • Chuang Xiao,
  • Rui Zhao,
  • Yuhuan Miao,
  • Dahui Liu

摘要

Artemisia argyi is a renowned Chinese medicinal herb extensively utilized for treating gynecological diseases. However, the application of bioreactor systems for the production of bioactive metabolites in A. argyi has yet to be explored. In this study, we established the callus cell suspension culture and genetic transformation systems for A. argyi for the first time. The optimal conditions for the culture system were determined to be an initial inoculation amount of 10%, a medium hormone ratio of 2.0 mg/L 2,4-D + 0.5 mg/L 6-BA + 1.0 mg/L NAA, a medium volume of 25 mL, a shaking speed of 140 rpm, and a light/dark cycle of 16 h/8 hours. Under these cultivation conditions, the cell fresh weight reached 146.98 g/L around the 20th day. Especially, the content of isochlorogenic acid A could reach to 321.06 mg/L, which is 258.92 times higher than that in vivo. Furthermore, we developed an efficient genetic transformation system for A. argyi by Agrobacterium tumefaciens strain EHA105, achieving a maximum transformation efficiency of 60% under conditions that included pre-culturing for 4 days, a bacterial concentration (OD600) of 1.2, an inoculation time of 15 min, and a co-cultivation period of 2 days. Using this system to overexpress RUBY gene, the content of betacyanin and betaxanthin in A. argyi callus could reach 535.79 mg/L and 640.90 mg/L, respectively. Based on the establishment of these two systems, we have devised a comprehensive and effective production strategy for active ingredients, which can be applied to the industrial production of bioactive compounds of A. argyi in the future.