Scholarlink

<p>Compared with callus, <i>Catharanthus roseus</i> (CAT) cambial meristematic cells (CMCs) exhibit significantly higher production of pharmacologically active terpenoid indole alkaloids (TIA), such as the anticancer drug vincristine and the antihypertensive drug ajmaline. Elicitors can significantly promote the biosynthesis of secondary metabolites in plant cells. However, the regulation of alkaloid biosynthesis in <i>C. roseus</i> CMCs by elicitors 2-Hydroxypropyl-β-cyclodextrin (H-β-CD) and 28-Homobrassinolide (HBL) is still unknown. Therefore, we established a new cambial meristematic cell line, named <i>C. roseus</i> CMCs-2, and investigated the effects of H-β-CD and HBL individually or in combination on the TIA content of CMCs in Erlenmeyer flasks (100, 250 and 500&#xa0;mL) or in a 5-L airlift bioreactor. CMCs cultured in the bioreactor and treated with 10.0&#xa0;g/L H-β-CD and 150&#xa0;μg/L HBL gave the highest yields of vindoline (4.89&#xa0;mg/L), catharanthine (2.49&#xa0;mg/L) vinblastine (3.96&#xa0;mg/L) and ajmalicine (3.87&#xa0;mg/L), concentrations that were 241, 277, 165 and 110% higher, respectively, than yields of CMCs cultured in 100-mL flasks without elicitors. These results indicated that CMCs cultured in the bioreactor exhibited enhanced production of vindoline, catharanthine, vinblastine and ajmalicine than those cultured in flasks. Quantitative RT-PCR showed that H-β-CD and HBL up-regulated transcription levels of genes related to the biosynthesis of TIAs. These results imply that H-β-CD and HBL can boost TIA production in CMCs, thereby indicating that <i>C. roseus</i> CMCs have considerable potential as an industrial platform for the production of bioactive TIAs.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Effects of 2-hydroxypropyl-β-cyclodextrin and 28-homobrassinolide on the physiological characteristics, gene expression and terpenoid indole alkaloid accumulation in Catharanthus roseus cambial meristematic cell cultures

  • 期刊论文

  • 发表日期：

    2025年4月29日

查看全文
  • Fangfang Fu,
  • Haihua Li,
  • Li Ling,
  • Jiahe Li,
  • Yifei Chen,
  • Weijie Hu,
  • Jiayu Li,
  • Lin Ou,
  • Zhaoxi Chen,
  • Jun Li,
  • Pengfei Zhou

摘要

Compared with callus, Catharanthus roseus (CAT) cambial meristematic cells (CMCs) exhibit significantly higher production of pharmacologically active terpenoid indole alkaloids (TIA), such as the anticancer drug vincristine and the antihypertensive drug ajmaline. Elicitors can significantly promote the biosynthesis of secondary metabolites in plant cells. However, the regulation of alkaloid biosynthesis in C. roseus CMCs by elicitors 2-Hydroxypropyl-β-cyclodextrin (H-β-CD) and 28-Homobrassinolide (HBL) is still unknown. Therefore, we established a new cambial meristematic cell line, named C. roseus CMCs-2, and investigated the effects of H-β-CD and HBL individually or in combination on the TIA content of CMCs in Erlenmeyer flasks (100, 250 and 500 mL) or in a 5-L airlift bioreactor. CMCs cultured in the bioreactor and treated with 10.0 g/L H-β-CD and 150 μg/L HBL gave the highest yields of vindoline (4.89 mg/L), catharanthine (2.49 mg/L) vinblastine (3.96 mg/L) and ajmalicine (3.87 mg/L), concentrations that were 241, 277, 165 and 110% higher, respectively, than yields of CMCs cultured in 100-mL flasks without elicitors. These results indicated that CMCs cultured in the bioreactor exhibited enhanced production of vindoline, catharanthine, vinblastine and ajmalicine than those cultured in flasks. Quantitative RT-PCR showed that H-β-CD and HBL up-regulated transcription levels of genes related to the biosynthesis of TIAs. These results imply that H-β-CD and HBL can boost TIA production in CMCs, thereby indicating that C. roseus CMCs have considerable potential as an industrial platform for the production of bioactive TIAs.