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<p>As one of the four major cut flowers globally, lily (<i>Lilium</i> spp.) has significant ornamental and economic value. However, the production and application of lily is particularly limited by its poor stress resistance and lack of self-propagation ability. Additionally, genetic transformation technology plays a crucial role in varietal improvement and has established itself as a cornerstone in plant functional genomics research, providing significant support to various plant enhancement programs. Thus, the objective of this study was to develop an effective lily regeneration system by testing various concentrations of plant growth regulators. The optimized genetic transformation system for tiger lily (<i>L. lancifolium</i>) was developed by determining the critical antibiotic concentration, bacterial solution concentration, and infection time. The results indicated that the optimal sterilization of tiger lily bulb explants was achieved by soaking them in 5% sodium hypochlorite for 10&#xa0;min. The combination of 6-Benzylaminopurine (6-BA) at 2.5&#xa0;mg/L and 1-Naphthaleneacetic acid (NAA) at 0.8&#xa0;mg/L effectively promoted the differentiation of adventitious buds. Additionally, a NAA concentration of 1.5&#xa0;mg/L was most beneficial for the rooting of adventitious buds. For the genetic transformation system, the optimal infection duration of 15&#xa0;min yielded the highest stable transformation rate of 47.1%. Consequently, the stress resistance gene <i>LlR3MYB</i> was homologously overexpressed in tiger lily and transgenic lines were successfully identified. This study offers valuable technical support for the improvement of reproductive capacity and the enhancement of conversion efficiency of tiger lily.</p>

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In vitro regeneration and Agrobacterium-mediated genetic transformation of Lilium lancifolium

  • 期刊论文

  • 发表日期：

    2025年9月11日

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  • Mingyue Li,
  • Heng Bi,
  • Yichao Zhu,
  • Qi Zhou,
  • Yubing Yong,
  • Wen Xing

摘要

As one of the four major cut flowers globally, lily (Lilium spp.) has significant ornamental and economic value. However, the production and application of lily is particularly limited by its poor stress resistance and lack of self-propagation ability. Additionally, genetic transformation technology plays a crucial role in varietal improvement and has established itself as a cornerstone in plant functional genomics research, providing significant support to various plant enhancement programs. Thus, the objective of this study was to develop an effective lily regeneration system by testing various concentrations of plant growth regulators. The optimized genetic transformation system for tiger lily (L. lancifolium) was developed by determining the critical antibiotic concentration, bacterial solution concentration, and infection time. The results indicated that the optimal sterilization of tiger lily bulb explants was achieved by soaking them in 5% sodium hypochlorite for 10 min. The combination of 6-Benzylaminopurine (6-BA) at 2.5 mg/L and 1-Naphthaleneacetic acid (NAA) at 0.8 mg/L effectively promoted the differentiation of adventitious buds. Additionally, a NAA concentration of 1.5 mg/L was most beneficial for the rooting of adventitious buds. For the genetic transformation system, the optimal infection duration of 15 min yielded the highest stable transformation rate of 47.1%. Consequently, the stress resistance gene LlR3MYB was homologously overexpressed in tiger lily and transgenic lines were successfully identified. This study offers valuable technical support for the improvement of reproductive capacity and the enhancement of conversion efficiency of tiger lily.