<p>Finger millet (<i>Eleusine coracana</i> (L.) Gaertn.), is an economically important cereal crop that is widely cultivated in the arid and semi-arid tropical areas of Asia and Africa. Owing to its short growing cycle, pest and drought resistance, it is a sustainable food crop in these regions. It is one of the several millets that not only can provide food security, but also offer nutritional benefits. Finger millet has a low glycemic index, is rich in dietary fiber, protein, amino acids and minerals, with the highest calcium content among all cereals. Genetic transformation of finger millets to improve traits like biotic and abiotic stress resistance is essential to meet the ever-growing food demand and is dependent on an optimized in-vitro regeneration protocol. The current review summarizes the various in-vitro regeneration studies reported for different varieties of finger millets. It provides a comprehensive understanding of the influence of genotype, explant, phytohormones, organic and inorganic additives on the regeneration potential of finger millets. Both organogenesis- and somatic embryogenesis-based regeneration approaches have been reported. From the available literature, it is evident that in-vitro regeneration requirements vary between different varieties and are highly genotype-dependent. Although regeneration protocols are available for many varieties, very few transformation studies have been carried out in finger millets. Optimizing a reproducible regeneration protocol for the more recently released biofortified varieties will boost genetic transformation and incorporation of agronomic traits like resistance to blast disease, lodging resistance and improved palatability.</p>