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<p><i>Melliodendron xylocarpum</i> is an endemic deciduous tree in China that has ornamental, oil, medicinal, and timber value. Conventional propagation methods (seedlings and cuttings) are characterized by low efficiency, long cycles, and time constraints. These limitations can be overcome by in vitro rapid propagation technology, making it an inevitable trend in the large-scale production of this species. This study used response surface methodology (RSM) to analyze the effects of five mineral salts, namely K₂SO₄, MgSO₄·7&#xa0;H₂O, KH₂PO₄, NH₄NO₃, and Ca(NO₃)₂·4&#xa0;H₂O, at concentration intervals ranging from 0.5 to 3.0 times their standard levels in woody plant medium (WPM) on the multiplication of <i>M. xylocarpum in vitro</i> shoots, aiming to optimize its medium formulation. The results showed that the multiplication of <i>M. xylocarpum in vitro</i> shoots was significantly influenced by two single factors, Ca(NO₃)₂·4&#xa0;H₂O and NH₄NO₃, as well as four interaction pairs: MgSO₄·7&#xa0;H₂O×KH₂PO₄, MgSO₄·7&#xa0;H₂O×Ca(NO₃)₂·4&#xa0;H₂O, KH₂PO₄×Ca(NO₃)₂·4&#xa0;H₂O, and NH₄NO₃×Ca(NO₃)₂·4&#xa0;H₂O. In contrast, other single mineral salts and interactions showed no significant effects. Through model prediction and experimental verification, the optimal concentration of the five mineral salts were 504.9&#xa0;mg/L K₂SO₄ (0.51×), 192.4&#xa0;mg/L MgSO₄·7&#xa0;H₂O (0.52×), 510&#xa0;mg/L KH₂PO₄ (3.00×), 200&#xa0;mg/L NH₄NO₃ (0.50×), and 1662.44&#xa0;mg/L Ca(NO₃)₂·4&#xa0;H₂O (2.99×). This study constructed a specialized multiplication medium for <i>M. xylocarpum</i> that optimized the in vitro rapid propagation technology system for <i>M. xylocarpum</i>, promoting its industrialization and large-scale production.</p>

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Optimization of Melliodendron xylocarpum multiplication medium formulation using response surface methodology

  • 期刊论文

  • 发表日期：

    2025年1月29日

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  • Yiting Gu,
  • Chen Wang,
  • Yifan Leng,
  • Ping Gao,
  • Min Li,
  • Shusheng Wen

摘要

Melliodendron xylocarpum is an endemic deciduous tree in China that has ornamental, oil, medicinal, and timber value. Conventional propagation methods (seedlings and cuttings) are characterized by low efficiency, long cycles, and time constraints. These limitations can be overcome by in vitro rapid propagation technology, making it an inevitable trend in the large-scale production of this species. This study used response surface methodology (RSM) to analyze the effects of five mineral salts, namely K₂SO₄, MgSO₄·7 H₂O, KH₂PO₄, NH₄NO₃, and Ca(NO₃)₂·4 H₂O, at concentration intervals ranging from 0.5 to 3.0 times their standard levels in woody plant medium (WPM) on the multiplication of M. xylocarpum in vitro shoots, aiming to optimize its medium formulation. The results showed that the multiplication of M. xylocarpum in vitro shoots was significantly influenced by two single factors, Ca(NO₃)₂·4 H₂O and NH₄NO₃, as well as four interaction pairs: MgSO₄·7 H₂O×KH₂PO₄, MgSO₄·7 H₂O×Ca(NO₃)₂·4 H₂O, KH₂PO₄×Ca(NO₃)₂·4 H₂O, and NH₄NO₃×Ca(NO₃)₂·4 H₂O. In contrast, other single mineral salts and interactions showed no significant effects. Through model prediction and experimental verification, the optimal concentration of the five mineral salts were 504.9 mg/L K₂SO₄ (0.51×), 192.4 mg/L MgSO₄·7 H₂O (0.52×), 510 mg/L KH₂PO₄ (3.00×), 200 mg/L NH₄NO₃ (0.50×), and 1662.44 mg/L Ca(NO₃)₂·4 H₂O (2.99×). This study constructed a specialized multiplication medium for M. xylocarpum that optimized the in vitro rapid propagation technology system for M. xylocarpum, promoting its industrialization and large-scale production.