<p>This research introduces a efficient approach to green chemistry by leveraging non-thermal plasma technology to create highly efficient, sustainable catalysts for dihydropyrimidone synthesis. By using natural biomass as a catalyst precursor, this study advances eco-friendly catalysis, reducing reliance on synthetic chemicals while harnessing renewable resources. The innovative plasma-assisted process, utilizing nitrogen and hydrogen gases, enables precise functionalization of the catalyst surface with carboxylic acid and amine groups eliminating toxic reagents and significantly lowering environmental impact. The resulting biomass-derived graphene oxide catalyst exhibited significant catalytic activity and selectivity, outperforming traditional chemically modified catalysts in the one-pot synthesis of dihydropyrimidone derivatives. Furthermore, it demonstrated exceptional stability, maintaining high efficiency across multiple reuse cycles without degradation. This work showcases the synergy between plasma technology and biomass conversion, providing a scalable, sustainable alternative for catalyst design. By integrating green principles with cutting-edge functionalization strategies, it sets a new benchmark for eco-conscious catalysis. The novel approach not only enhances reaction efficiency but also aligns with global sustainability goals, paving the way for cleaner, greener chemical processes. This research opens exciting avenues for next-generation catalyst development, merging innovation with environmental responsibility.</p> Graphical abstract <p></p>