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<p>Mugwort is derived from the dried leaves of <i>Artemisia argyi</i> Lévl. et Vant. (Asteraceae). Flavonoids represent a major class of bioactive constituents in <i>A. argyi</i> leaves and contribute significantly to its pharmacological activities. However, the regulatory mechanisms of flavonoid biosynthesis in <i>A. argyi</i> remain unclear. In this study, one R2R3-MYB family transcription factor, <i>AaMYB57</i>, was cloned from <i>A. argyi</i> leaves. The open reading frame of <i>AaMYB57</i> was 993&#xa0;bp, encoding 330 amino acids. The predicted relative molecular weight was 37,720.08, the theoretical isoelectric point was 8.44, the total number of atoms was 5224, the aliphatic index was 65.91, the instability index was 50.68, and the hydrophilicity coefficient was -0.840, suggesting that the <i>AaMYB57</i> protein is a hydrophilic protein. <i>AaMYB57</i> was localized in the nucleus. Sequence alignment results indicated that <i>AaMYB57</i> had the highest homology with <i>AanMYB1</i> from <i>A. annua</i>. The plant expression vector pBWA(V)HS-AaMYB57-Glosgfp was constructed using the double enzyme digestion method, and overexpression lines of the <i>AaMYB57</i> in tobacco were obtained through Agrobacterium-mediated transformation. Compared with wild-type tobacco, the total flavonoid and rutin contents were higher in the <i>AaMYB57</i> overexpression lines. Meanwhile, qRT-PCR analysis showed that the expression levels of genes involved in the flavonoid synthesis pathway, such as <i>4CL</i>, <i>CHI</i>, and <i>F3H</i>, were upregulated in the overexpression lines. It is preliminarily speculated that <i>AaMYB57</i> is involved in regulating the expression of enzyme genes in the flavonoid biosynthesis pathway, thereby affecting flavonoid synthesis in <i>A. argyi</i> leaves. This study establishes a foundation for further understanding the molecular mechanisms underlying flavonoid biosynthesis in <i>A. argyi</i>.</p>

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Cloning and Functional Characterization of R2R3-MYB Transcription Factor AaMYB57 from Artemisia argyi

  • 期刊论文

  • 发表日期：

    2026年6月25日

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  • Zhanhu Cui,
  • Chunyan Miao,
  • Mengzhi Li,
  • Qian Zhao,
  • Zhongyi Zhang,
  • Xianzhang Huang

摘要

Mugwort is derived from the dried leaves of Artemisia argyi Lévl. et Vant. (Asteraceae). Flavonoids represent a major class of bioactive constituents in A. argyi leaves and contribute significantly to its pharmacological activities. However, the regulatory mechanisms of flavonoid biosynthesis in A. argyi remain unclear. In this study, one R2R3-MYB family transcription factor, AaMYB57, was cloned from A. argyi leaves. The open reading frame of AaMYB57 was 993 bp, encoding 330 amino acids. The predicted relative molecular weight was 37,720.08, the theoretical isoelectric point was 8.44, the total number of atoms was 5224, the aliphatic index was 65.91, the instability index was 50.68, and the hydrophilicity coefficient was -0.840, suggesting that the AaMYB57 protein is a hydrophilic protein. AaMYB57 was localized in the nucleus. Sequence alignment results indicated that AaMYB57 had the highest homology with AanMYB1 from A. annua. The plant expression vector pBWA(V)HS-AaMYB57-Glosgfp was constructed using the double enzyme digestion method, and overexpression lines of the AaMYB57 in tobacco were obtained through Agrobacterium-mediated transformation. Compared with wild-type tobacco, the total flavonoid and rutin contents were higher in the AaMYB57 overexpression lines. Meanwhile, qRT-PCR analysis showed that the expression levels of genes involved in the flavonoid synthesis pathway, such as 4CL, CHI, and F3H, were upregulated in the overexpression lines. It is preliminarily speculated that AaMYB57 is involved in regulating the expression of enzyme genes in the flavonoid biosynthesis pathway, thereby affecting flavonoid synthesis in A. argyi leaves. This study establishes a foundation for further understanding the molecular mechanisms underlying flavonoid biosynthesis in A. argyi.