<p>Okra (<i>Abelmoschus esculentus</i> L.) is a vegetable crop of global importance because of its remarkable nutritional and medicinal qualities. Despite its importance, molecular-level research on its genetic diversity is limited in Iraqi Kurdistan. Therefore, assessing genetic diversity is crucial for the strategic development and selection of superior genotypes of okra. The aim of this study was to examine the genetic variation between 58 okra genotypes originating from local farmers and imported varieties using conserved DNA-derived polymorphism (CDDP), intersimple sequence repeat (ISSR), and start codon target (SCoT). A total of 301 scorable polymorphic bands were generated from three types of markers. The mean PIC values for ISSR, SCoT, and CDDP were 0.36, 0.28, and 0.33, respectively. Notably, the markers: UBC-846 (0.40), SCOT19 (0.47), and WRKYF1 (0.42) highlighted highly informative markers. The highest average values of the Shannon index (0.47) and expected heterozygosity (0.32) were recorded by the CDDP markers, followed by the SCoT markers. Cluster analysis and structure analysis divided the 58 genotypes into two primary clusters for ISSR and CDDP and three primary clusters for SCoT markers. Analysis of molecular variance revealed high percentages of total genetic variation within the population for all markers. The genetic identity analysis of the Nei population revealed a high degree of similarity between the populations. These results show that the ISSR, SCoT, and CDDP markers are effective in the assessment of the genetic diversity of okra. The identified genetically diverse parental groups may assist breeders in developing new abiotic and biotic stress-tolerant okra varieties.</p>