Characterization and Expression of the S-nitrosoglutathione Reductase (GSNOR) Gene from Jatropha curcas in Response to Azelaic Acid
摘要
The 1421 bp gene encoding S-nitrosoglutathione reductase (GSNOR) was successfully cloned and characterized from Jatropha curcas. The gene contains an open reading frame (ORF) of 1140 base pairs, encoding a polypeptide of 379 amino acids. Expression analysis revealed that JcGSNOR is constitutively expressed across all major organs of Jatropha curcas. To further investigate the role of JcGSNOR in systemic acquired resistance (SAR) and its molecular response to azelaic acid, a key SAR signaling molecule, the expression patterns of GSNOR under exogenous azelaic acid treatment were examined. Expression levels of GSNOR in both Zea mays and Jatropha curcas decreased with increasing concentrations of azelaic acid up to 600 μg·mL−1. Notably, while ZmGSNOR expression continued declining, JcGSNOR sharply increased at the 800 μg·mL−1 treatment, suggesting a species-specific regulatory response. Additionally, JcGSNOR was heterologously expressed in Saccharomyces cerevisiae and in the gsnor mutant of Arabidopsis thaliana. Our findings indicate that JcGSNOR enhances GSNOR enzymatic activity in S. cerevisiae, although it has minimal impact on yeast growth. Transgenic Arabidopsis thaliana expressing JcGSNOR demonstrated an improved tolerance to exogenous azelaic acid. This study provides the first characterization of GSNOR from Jatropha curcas and its response to azelaic acid. Our findings suggest that JcGSNOR contributes to defense-related signaling and may hold potential for enhancing plant resistance. These results deepen our understanding of the function of GSNOR in SAR signaling.