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<p>Pepino (<i>Solanum muricatum</i>) is an emerging and unexplored solanaceous fruit crop with potential for nutritional and breeding advancements. These genotypes were collected from different locations across Pakistan. The current investigation assessed genetic diversity among ten pepino genotypes on the basis of morphological, biochemical, and ISSR markers to identify a superior genotype. Results revealed that there is significant variation exhibited among genotypes in morphological traits, as plant height ranged from 67.9 to 88.1&#xa0;cm in G9 and G7 correspondingly, and fruit weight ranged from 139.5 to 178.3&#xa0;g in G9 and G7, respectively. From the results, it was observed that the G7 was the morphologically batter genotype. Findings of biochemical analysis revealed that maximum TSS (11.0°Brix), total phenolics (66.5&#xa0;mg GAE g⁻<sup>1</sup> FW), and total carotenoids (2.4&#xa0;mg/100&#xa0;g FW) were estimated in G7, while vitamin C content was highest (36.0&#xa0;mg 100&#xa0;g⁻<sup>1</sup> FW) in G9. Conclusively, G7 was the batter genotype as it gained higher values for biochemical traits like highest sweetness, phenolic content, and carotenoids among the genotypes, whereas G9 was leading in terms of elevated ascorbic acid content but lower overall fruit quality. Around 3–6 loci were amplified for each ISSR primer, and polymorphic information content (PIC) values ranged from 0.116 to 0.399. A significant polymorphism (65.85%) was displayed by primers UBC-812, UBC-815, and UBC-818, and considered most informative. Three main groups were constructed among the genotypes based on Clustering and STRUCTURE analyses, where group I included G8, G2, G10, G5, and G1 genotypes, group II comprised only two genotypes (G7 and G4), and group III contained G9, G3, and G6. Overall, integrating morphological, biochemical, and molecular data confirmed significant diversity within pepino germplasm.</p>

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Genetic Diversity and Allelic Admixture Among Pepino (Solanum muricatum) Genotypes Using Morphological, Biochemical, and Molecular Insights

  • 期刊论文

  • 发表日期：

    2026年4月23日

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  • Adel A. Rezk,
  • Maryam,
  • Othman Al-Dossary,
  • Hossam S. El- Beltagi,
  • Mohamed Aldaej,
  • Bader Y. Alsubaie,
  • Tarek A. W. Shalaby,
  • Nora M. Al Aboud,
  • Ishtiaq Ahmad

摘要

Pepino (Solanum muricatum) is an emerging and unexplored solanaceous fruit crop with potential for nutritional and breeding advancements. These genotypes were collected from different locations across Pakistan. The current investigation assessed genetic diversity among ten pepino genotypes on the basis of morphological, biochemical, and ISSR markers to identify a superior genotype. Results revealed that there is significant variation exhibited among genotypes in morphological traits, as plant height ranged from 67.9 to 88.1 cm in G9 and G7 correspondingly, and fruit weight ranged from 139.5 to 178.3 g in G9 and G7, respectively. From the results, it was observed that the G7 was the morphologically batter genotype. Findings of biochemical analysis revealed that maximum TSS (11.0°Brix), total phenolics (66.5 mg GAE g⁻1 FW), and total carotenoids (2.4 mg/100 g FW) were estimated in G7, while vitamin C content was highest (36.0 mg 100 g⁻1 FW) in G9. Conclusively, G7 was the batter genotype as it gained higher values for biochemical traits like highest sweetness, phenolic content, and carotenoids among the genotypes, whereas G9 was leading in terms of elevated ascorbic acid content but lower overall fruit quality. Around 3–6 loci were amplified for each ISSR primer, and polymorphic information content (PIC) values ranged from 0.116 to 0.399. A significant polymorphism (65.85%) was displayed by primers UBC-812, UBC-815, and UBC-818, and considered most informative. Three main groups were constructed among the genotypes based on Clustering and STRUCTURE analyses, where group I included G8, G2, G10, G5, and G1 genotypes, group II comprised only two genotypes (G7 and G4), and group III contained G9, G3, and G6. Overall, integrating morphological, biochemical, and molecular data confirmed significant diversity within pepino germplasm.