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<p>Cut rhododendron flowers are adored for their ornamental value. The regulation mechanism of molecules related to the senescence signal of abscisic acid (ABA) should be studied in cut rhododendrons to improve their postharvest value. The endogenous ABA content of <i>Rhododendron simsii</i>, <i>R</i>. <i>pulchrum</i>, <i>R</i>. <i>hybridum</i> ‘Hong Shanhu’, and <i>R</i>. <i>obtusum</i> ‘Yanzhi Mi’ was monitored during vase life. The effects of 0.2&#xa0;mM ABA and sodium tungstate (0.1&#xa0;mM Na<sub>2</sub>WO<sub>4</sub>) on the postharvest value were assessed. The endogenous ABA content in petals showed three distinct metabolic patterns during vase life: first decreasing then increasing (V-shaped), rising then falling before rising again (N-shaped), and the late-stage rising type. In the <i>R</i>. <i>simsii</i> genome, 32 genes related to ABA biosynthesis and catabolism were identified, and 27 genes were identified in ‘Hong Shanhu’ transcriptome data. The relative expression levels of key genes, including <i>NCED2</i>, <i>CYP707A3</i>, <i>CYP707A7</i>, <i>BG2</i>, and <i>BG9</i>, were measured using qPCR. The expression trends of <i>RsNCED2</i> and its homologous gene <i>RhsNCED2</i> were consistent with the change in the ABA content. <i>NCED2</i> played a key role in ABA biosynthesis in cut rhododendrons. The expression levels of <i>RsCYP707A7</i> and <i>RhsCYP707A7</i> were both significantly downregulated at SV, consistent with the high ABA levels of the two cut rhododendrons. Promoter cis-acting element analysis showed that the expression of ABA biosynthesis and catabolism genes was regulated by ABA and other hormones and abiotic stressors. Our findings help explain the aging mechanisms of cut rhododendrons and provide valuable insights for future studies on the functions of ABA-related genes.</p>

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Effect of ABA on Senescence of Cut Rhododendron Flowers and the Identification and Analysis of Genes Related to ABA Biosynthesis and Catabolism

  • 期刊论文

  • 发表日期：

    2026年3月27日

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  • Shenling Wang,
  • Zhili Jiang,
  • Fanyu Zeng,
  • Xingmin Geng

摘要

Cut rhododendron flowers are adored for their ornamental value. The regulation mechanism of molecules related to the senescence signal of abscisic acid (ABA) should be studied in cut rhododendrons to improve their postharvest value. The endogenous ABA content of Rhododendron simsii, R. pulchrum, R. hybridum ‘Hong Shanhu’, and R. obtusum ‘Yanzhi Mi’ was monitored during vase life. The effects of 0.2 mM ABA and sodium tungstate (0.1 mM Na2WO4) on the postharvest value were assessed. The endogenous ABA content in petals showed three distinct metabolic patterns during vase life: first decreasing then increasing (V-shaped), rising then falling before rising again (N-shaped), and the late-stage rising type. In the R. simsii genome, 32 genes related to ABA biosynthesis and catabolism were identified, and 27 genes were identified in ‘Hong Shanhu’ transcriptome data. The relative expression levels of key genes, including NCED2, CYP707A3, CYP707A7, BG2, and BG9, were measured using qPCR. The expression trends of RsNCED2 and its homologous gene RhsNCED2 were consistent with the change in the ABA content. NCED2 played a key role in ABA biosynthesis in cut rhododendrons. The expression levels of RsCYP707A7 and RhsCYP707A7 were both significantly downregulated at SV, consistent with the high ABA levels of the two cut rhododendrons. Promoter cis-acting element analysis showed that the expression of ABA biosynthesis and catabolism genes was regulated by ABA and other hormones and abiotic stressors. Our findings help explain the aging mechanisms of cut rhododendrons and provide valuable insights for future studies on the functions of ABA-related genes.