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<p>The <i>FtsH</i> protease, a member of the AAA + protease family, plays an essential role in plant growth, development, and stress resistance. However, research on <i>FtsH</i> proteases in tobacco remains limited. This study aims to elucidate the function of the <i>NtFtsH7</i> gene in regulating tobacco growth and development, as well as stress responses. We performed an extensive bioinformatic analysis of the <i>NtFtsH7</i> gene locus in tobacco, including investigations into its tissue-specific expression patterns and responses to abiotic stress. Furthermore, we validated the enhanced tolerance to low-temperature and drought stress in tobacco plants overexpressing <i>NtFtsH7</i>. Our results show that the <i>NtFtsH7</i> protein is an unstable, hydrophilic, basic protein localized to the endoplasmic reticulum. Cis-acting elements identified in its promoter region are associated with hormones, growth, development, and abiotic stress. Expression analysis revealed that <i>NtFtsH7</i> expression was highest in leaves, followed by flowers and stems, and lowest in roots. Moreover, <i>NtFtsH7</i> was significantly upregulated under various stress conditions and following treatment with three types of hormones. <i>NtFtsH7</i> overexpressing tobacco plants exhibited markedly enhanced tolerance to both low-temperature and drought stress. This study establishes a foundation for further understanding the molecular mechanisms underlying the stress response regulated by <i>NtFtsH7</i> in tobacco.</p>

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Cloning and Functional Analysis of the NtFtsH7 Gene in Tobacco under Stress Conditions

  • 期刊论文

  • 发表日期：

    2026年3月19日

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  • Tao Long,
  • Tianxiunan Pu,
  • Jie Tan,
  • Yuanshuai Shi,
  • Yang Liu

摘要

The FtsH protease, a member of the AAA + protease family, plays an essential role in plant growth, development, and stress resistance. However, research on FtsH proteases in tobacco remains limited. This study aims to elucidate the function of the NtFtsH7 gene in regulating tobacco growth and development, as well as stress responses. We performed an extensive bioinformatic analysis of the NtFtsH7 gene locus in tobacco, including investigations into its tissue-specific expression patterns and responses to abiotic stress. Furthermore, we validated the enhanced tolerance to low-temperature and drought stress in tobacco plants overexpressing NtFtsH7. Our results show that the NtFtsH7 protein is an unstable, hydrophilic, basic protein localized to the endoplasmic reticulum. Cis-acting elements identified in its promoter region are associated with hormones, growth, development, and abiotic stress. Expression analysis revealed that NtFtsH7 expression was highest in leaves, followed by flowers and stems, and lowest in roots. Moreover, NtFtsH7 was significantly upregulated under various stress conditions and following treatment with three types of hormones. NtFtsH7 overexpressing tobacco plants exhibited markedly enhanced tolerance to both low-temperature and drought stress. This study establishes a foundation for further understanding the molecular mechanisms underlying the stress response regulated by NtFtsH7 in tobacco.