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<p>Widely distributed from East Asia to Australia, <i>Rubus parvifolius</i> is the only known wild <i>Rubus</i> species that naturally grows throughout the Japanese Archipelago, including small southern islands. <i>R. parvifolius</i> has been attracting attention as a genetic resource in Asia for breeding raspberries with enhanced tolerance to environmental stresses. Amami Oshima and Kakeroma, two of Japan’s Amami Islands, exhibit high plant diversity due to significant species endemism, reflecting the islands' isolated biogeographical history. This provides a rationale for focusing on Amami Oshima and Kakeroma Islands as a suitable system for studying wild <i>Rubus</i> species. In this study, we conducted ploidy assessments and analyzed Simple Sequence Repeat (SSR) markers in 24 accessions of <i>R. parvifolius</i> and other wild <i>Rubus</i> species (<i>R. grayanus</i> and <i>R. croceacanthus</i>) from those islands. Ploidy evaluation using flow cytometry and chromosome observation revealed that 11 of the 24 accessions (45.8%) were triploid. In addition, 52 SSR markers were employed, of which 20 were selected for analysis. Principal coordinate analysis (PCoA) using these SSR markers showed that diploid and triploid individuals did not form distinct clusters. Although this study was preliminary and based on a limited sample size, a relatively frequent occurrence of polyploid individuals was observed among the <i>R. parvifolius</i> accessions examined from Amami Oshima and Kakeroma. Our results provide baseline information on the occurrence of polyploid individuals in wild species in island systems.</p>

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Unexpected Occurrence of Triploids and Preliminary Assessment of Genetic Relationships Using SSR Markers in Rubus parvifolius on Amami Oshima and Kakeroma, Japan

  • 期刊论文

  • 发表日期：

    2026年2月24日

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  • Saki Toshima,
  • Kodai Egashira,
  • Makiko Mimura,
  • Yuichi Saeki,
  • Tomonari Hirano,
  • Hisato Kunitake

摘要

Widely distributed from East Asia to Australia, Rubus parvifolius is the only known wild Rubus species that naturally grows throughout the Japanese Archipelago, including small southern islands. R. parvifolius has been attracting attention as a genetic resource in Asia for breeding raspberries with enhanced tolerance to environmental stresses. Amami Oshima and Kakeroma, two of Japan’s Amami Islands, exhibit high plant diversity due to significant species endemism, reflecting the islands' isolated biogeographical history. This provides a rationale for focusing on Amami Oshima and Kakeroma Islands as a suitable system for studying wild Rubus species. In this study, we conducted ploidy assessments and analyzed Simple Sequence Repeat (SSR) markers in 24 accessions of R. parvifolius and other wild Rubus species (R. grayanus and R. croceacanthus) from those islands. Ploidy evaluation using flow cytometry and chromosome observation revealed that 11 of the 24 accessions (45.8%) were triploid. In addition, 52 SSR markers were employed, of which 20 were selected for analysis. Principal coordinate analysis (PCoA) using these SSR markers showed that diploid and triploid individuals did not form distinct clusters. Although this study was preliminary and based on a limited sample size, a relatively frequent occurrence of polyploid individuals was observed among the R. parvifolius accessions examined from Amami Oshima and Kakeroma. Our results provide baseline information on the occurrence of polyploid individuals in wild species in island systems.