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<p>Losses associated with fungal pathogens that are deteriorating the citrus fruit qualities in different citrus-growing regions of Pakistan were investigated. However, this problem has not been addressed in Khanpur, the capital of the Haripur district, which is a major citrus-growing region in Pakistan. Citrus fruit quality and quantity deteriorate due to various fungi impairing their quiet standards, resulting in economic losses. The current investigation involves three steps: morphological, pathological, and molecular identification of the fungal pathogens linked to citrus fruit diseases. Firstly, the pathogens from infected fruit samples were isolated by culturing them on potato dextrose agar (PDA) for morphological characterization. Secondly, the 5.8S rDNA molecular sequencing was used to identify the crucial species at the molecular level. The results showed these fungal pathogens belonged to the seven important genera of <i>Colletotrichum</i>, <i>Alternaria</i>, <i>Aspergillus</i>, <i>Botryosphaeria</i>, <i>Lasiodiplodia</i>, <i>Penicillium</i>, and <i>Fusarium</i>. And thirdly, fungal strains were subjected to pathogenicity assays to confirm the virulence and pathogen association with the citrus fruit diseases followed by re-isolation. Current work associated with the comprehension of disease development points towards effective managerial practices in the form of a cost-effective spray program. Furthermore, the findings indicate the need for biological and physical pre-harvest management interventions to increase storage and marketability.</p>

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Morphological and Molecular Characterization of Fungal Pathogens Associated with Citrus Fruit

  • 期刊论文

  • 发表日期：

    2025年12月15日

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  • Iram Zaheer,
  • Shazia Iram,
  • Sajjad Hyder,
  • Amjad Shahzad Gondal,
  • Ghulam Murtaza,
  • Rashid Iqbal,
  • Mohamed Taha Yassin

摘要

Losses associated with fungal pathogens that are deteriorating the citrus fruit qualities in different citrus-growing regions of Pakistan were investigated. However, this problem has not been addressed in Khanpur, the capital of the Haripur district, which is a major citrus-growing region in Pakistan. Citrus fruit quality and quantity deteriorate due to various fungi impairing their quiet standards, resulting in economic losses. The current investigation involves three steps: morphological, pathological, and molecular identification of the fungal pathogens linked to citrus fruit diseases. Firstly, the pathogens from infected fruit samples were isolated by culturing them on potato dextrose agar (PDA) for morphological characterization. Secondly, the 5.8S rDNA molecular sequencing was used to identify the crucial species at the molecular level. The results showed these fungal pathogens belonged to the seven important genera of Colletotrichum, Alternaria, Aspergillus, Botryosphaeria, Lasiodiplodia, Penicillium, and Fusarium. And thirdly, fungal strains were subjected to pathogenicity assays to confirm the virulence and pathogen association with the citrus fruit diseases followed by re-isolation. Current work associated with the comprehension of disease development points towards effective managerial practices in the form of a cost-effective spray program. Furthermore, the findings indicate the need for biological and physical pre-harvest management interventions to increase storage and marketability.