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<p>RING-type E3 ubiquitin ligase is involved in the degradation of stress-related proteins and plays an important role in plant response and adaptation to abiotic stresses such as drought and salt. In this study, the full length of the <i>PnLOG2</i> coding region of the E3 ubiquitin ligase gene was cloned from <i>Ipomoea purpurea</i> Lam., and the expression characteristics of the <i>PNLOG2</i> gene under different tissues, drought, salt and ABA treatment were analyzed. In addition, drought, salt, and ABA treatment significantly upregulated the expression of the <i>PnLOG2</i> gene. Functional verification in yeast showed that the <i>PnLOG2</i> gene significantly improved the resistance of transgenic yeast to drought and salt stress. <i>PnLOG2</i> overexpression in <i>Arabidopsis thaliana</i> was further constructed. Under drought and salt stress, overexpression of <i>Arabidopsis</i> significantly increased seed germination rate and seedling survival rate compared with WT. The fresh weight and chlorophyll content of transgenic <i>Arabidopsis thaliana</i> increased, while the MDA content decreased. In addition, after exogenous ABA treatment, the sensitivity of <i>PnLOG2</i> overexpressed plants to ABA was enhanced, and the expression levels of <i>AtNCED</i>, <i>AtRD20</i>, <i>AtRAB18</i>, and <i>AtRD19B</i> in overexpressed plants were significantly different from those in WT. These results indicated that the <i>PnLOG2</i> gene might be involved in ABA signal transduction, thereby enhancing the resistance of transgenic plants to drought and salt stress. This study provides a theoretical basis for the role of RING-type E3 ubiquitin ligase in plant response to abiotic stress.</p>

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RING-Type E3 Ubiquitin Ligase Gene PnLOG2 Increased Arabidopsis Drought and Salt Stress Resistance

  • 期刊论文

  • 发表日期：

    2025年12月1日

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  • Jing Guo,
  • Yanlong Dong,
  • Yexi Gao,
  • Shengnan Hou,
  • Geli Wang,
  • Meiyu Zhang,
  • Chao Chen,
  • Xiaoxia Jin

摘要

RING-type E3 ubiquitin ligase is involved in the degradation of stress-related proteins and plays an important role in plant response and adaptation to abiotic stresses such as drought and salt. In this study, the full length of the PnLOG2 coding region of the E3 ubiquitin ligase gene was cloned from Ipomoea purpurea Lam., and the expression characteristics of the PNLOG2 gene under different tissues, drought, salt and ABA treatment were analyzed. In addition, drought, salt, and ABA treatment significantly upregulated the expression of the PnLOG2 gene. Functional verification in yeast showed that the PnLOG2 gene significantly improved the resistance of transgenic yeast to drought and salt stress. PnLOG2 overexpression in Arabidopsis thaliana was further constructed. Under drought and salt stress, overexpression of Arabidopsis significantly increased seed germination rate and seedling survival rate compared with WT. The fresh weight and chlorophyll content of transgenic Arabidopsis thaliana increased, while the MDA content decreased. In addition, after exogenous ABA treatment, the sensitivity of PnLOG2 overexpressed plants to ABA was enhanced, and the expression levels of AtNCED, AtRD20, AtRAB18, and AtRD19B in overexpressed plants were significantly different from those in WT. These results indicated that the PnLOG2 gene might be involved in ABA signal transduction, thereby enhancing the resistance of transgenic plants to drought and salt stress. This study provides a theoretical basis for the role of RING-type E3 ubiquitin ligase in plant response to abiotic stress.