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<p>Yam is widely loved for its medicinal, health benefits and delicious taste. This study sequenced transcriptomes of different parts of <i>Dioscorea alata</i> roots using Illumina HiSeq 2000, obtaining 47,547 unigenes (average length 1803&#xa0;bp, N50 2605&#xa0;bp) via de novo assembly. It detected 1162, 2754 and 3607 DEGs in S1-vs.-S2, S1-vs.-S3 and S1-vs.-S4, with 416 DEGs shared across all groups. Functional annotation showed genes may participate in cellular processes, biological regulation, metabolism, stimulus response, etc. Detailed analysis covered DEGs related to starch and sucrose metabolism, as well as classification of TFs in unigenes and DEGs. The transcriptome resources here will aid gene discovery and utilization, boosting <i>D. alata</i> molecular breeding.</p>

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Transcriptomic Profiling of Dioscorea alata Roots Highlights Starch and Sucrose Metabolism Pathways

  • 期刊论文

  • 发表日期：

    2025年9月29日

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  • Peilong Wang,
  • Wan Xu,
  • Dandan Kong,
  • Zhuang Zhou

摘要

Yam is widely loved for its medicinal, health benefits and delicious taste. This study sequenced transcriptomes of different parts of Dioscorea alata roots using Illumina HiSeq 2000, obtaining 47,547 unigenes (average length 1803 bp, N50 2605 bp) via de novo assembly. It detected 1162, 2754 and 3607 DEGs in S1-vs.-S2, S1-vs.-S3 and S1-vs.-S4, with 416 DEGs shared across all groups. Functional annotation showed genes may participate in cellular processes, biological regulation, metabolism, stimulus response, etc. Detailed analysis covered DEGs related to starch and sucrose metabolism, as well as classification of TFs in unigenes and DEGs. The transcriptome resources here will aid gene discovery and utilization, boosting D. alata molecular breeding.