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Abstract <p>The escalating global population and adverse effects of climate change are placing unprecedented pressure on agriculture to produce food commodities in greater quantities. Abiotic stresses, including drought, salinity, and extreme temperatures, are primary constraints that diminish crop yields and threaten global food security. Traditional breeding and biotechnological approaches have contributed to the creation of tolerant cultivars, but they are often time-consuming, labour-intensive, and less efficient. CRISPR/Cas9, a powerful genome-editing tool, has emerged as a promising alternative due to its precision, efficiency, and capability to induce mutations in targeted regions. This review provides an overview of plant responses to abiotic stress, emphasizing the roles of key genes, signalling pathways, and transcriptional regulators involved in the process. The review also discusses how CRISPR/Cas9 technology has been employed in various crops to enhance tolerance to stresses such as drought, salinity, heat, and cold by editing stress-responsive genes. Recent advancements in CRISPR-based editing, including base editing, prime editing, and multiplex genome editing (MGE), are also highlighted. Despite its immense potential, challenges such as off-target effects, genome complexity, and regulatory hurdles must be addressed. CRISPR/Cas9 represents an innovative method for crop improvement, paving the way for the development of resilient crop cultivars adapted to changing climatic conditions.</p> Graphical Abstract <p></p>

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CRISPR/Cas9-Mediated Genome Editing for Abiotic Stress Tolerance in Crops: Current Advances and Future Prospects

  • 期刊论文

  • 发表日期：

    2025年7月11日

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  • Ajithkumar V.,
  • Soni K. B.,
  • Swapna Alex,
  • Anuradha T.,
  • Rehna Augustine,
  • Manju R. V.

摘要

Abstract

The escalating global population and adverse effects of climate change are placing unprecedented pressure on agriculture to produce food commodities in greater quantities. Abiotic stresses, including drought, salinity, and extreme temperatures, are primary constraints that diminish crop yields and threaten global food security. Traditional breeding and biotechnological approaches have contributed to the creation of tolerant cultivars, but they are often time-consuming, labour-intensive, and less efficient. CRISPR/Cas9, a powerful genome-editing tool, has emerged as a promising alternative due to its precision, efficiency, and capability to induce mutations in targeted regions. This review provides an overview of plant responses to abiotic stress, emphasizing the roles of key genes, signalling pathways, and transcriptional regulators involved in the process. The review also discusses how CRISPR/Cas9 technology has been employed in various crops to enhance tolerance to stresses such as drought, salinity, heat, and cold by editing stress-responsive genes. Recent advancements in CRISPR-based editing, including base editing, prime editing, and multiplex genome editing (MGE), are also highlighted. Despite its immense potential, challenges such as off-target effects, genome complexity, and regulatory hurdles must be addressed. CRISPR/Cas9 represents an innovative method for crop improvement, paving the way for the development of resilient crop cultivars adapted to changing climatic conditions.

Graphical Abstract