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<p>In East Asia, <i>Perilla</i> is highly valued for its nutritional value and traditional culinary uses, especially in South Korea and Japan, where it is extensively grown for perilla oil and consumed as a leafy vegetable. To support genetic and breeding research on <i>Perilla</i>, various molecular marker technologies are essential. However, there remains a shortage of simple sequence repeat (SSR) primer sets for studying genetic variation among <i>P. frutescens</i> var. <i>frutescens</i> (PF) and <i>P. frutescens</i> var. <i>crispa</i> (PC) cultivars from South Korea and Japan. In this study, 600 SSR primer sets were newly developed using RNA-sequencing and then evaluated across four <i>Perilla</i> accessions and classified into four distinct categories: none, monomorphic, multi, and polymorphic band patterns. Of these, 173 (28.8%) showed polymorphic bands, and 70 of these SSR primers were used to study genetic diversity, genetic relationships, and structure population among 25 <i>Perilla</i> cultivars collected from South Korea and Japan. A total of 223 alleles were detected across all loci, with an average of 3.2 alleles per locus. Gene diversity (GD) ranged from 0.077 to 0.842, with an average of 0.494. The average GD values were 0.373 in PF cultivars and 0.383 in PC cultivars. Analysis of population structure and genetic relationships using SSR markers showed that, except for one cultivar, there were clear distinctions between PF and PC cultivars. Therefore, the newly developed SSR primers identified in this study can serve as valuable tools for detecting genetic variation and elucidating relationships between PF and PC cultivars.</p>

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Genetic Variation among Cultivars of Perilla Frutescens (L.) Britton Using new Developed Perilla SSR Markers

  • 期刊论文

  • 发表日期：

    2025年7月2日

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  • Da Hyeon Lee,
  • Hyeon Park,
  • Tae Hyeon Heo,
  • Jungeun Cho,
  • Ju Kyong Lee

摘要

In East Asia, Perilla is highly valued for its nutritional value and traditional culinary uses, especially in South Korea and Japan, where it is extensively grown for perilla oil and consumed as a leafy vegetable. To support genetic and breeding research on Perilla, various molecular marker technologies are essential. However, there remains a shortage of simple sequence repeat (SSR) primer sets for studying genetic variation among P. frutescens var. frutescens (PF) and P. frutescens var. crispa (PC) cultivars from South Korea and Japan. In this study, 600 SSR primer sets were newly developed using RNA-sequencing and then evaluated across four Perilla accessions and classified into four distinct categories: none, monomorphic, multi, and polymorphic band patterns. Of these, 173 (28.8%) showed polymorphic bands, and 70 of these SSR primers were used to study genetic diversity, genetic relationships, and structure population among 25 Perilla cultivars collected from South Korea and Japan. A total of 223 alleles were detected across all loci, with an average of 3.2 alleles per locus. Gene diversity (GD) ranged from 0.077 to 0.842, with an average of 0.494. The average GD values were 0.373 in PF cultivars and 0.383 in PC cultivars. Analysis of population structure and genetic relationships using SSR markers showed that, except for one cultivar, there were clear distinctions between PF and PC cultivars. Therefore, the newly developed SSR primers identified in this study can serve as valuable tools for detecting genetic variation and elucidating relationships between PF and PC cultivars.