Scholarlink

Background and Aims <p>Salt stress is a major constraint limiting crop productivity worldwide. Among the sustainable and environmentally friendly strategies proposed to mitigate salt-induced reductions in crop growth and yield, the exogenous application of proline and silicon (Si) has attracted increasing attention.</p> Methods <p>Here, we investigated the effects of the individual and combined application of 20&#xa0;mM proline and 1.24&#xa0;mM Si on <i>Medicago truncatula,</i> a model legume, exposed to salt stress.</p> Results <p>The results showed that continuous exposure to 120&#xa0;mM NaCl significantly impaired plant growth indexes, including shoot and root dry weight, plant height, leaf number and area. Exogenous application of either proline or Si partially alleviated the detrimental effects of NaCl on these growth indexes. Notably, the combined application of proline and Si conferred greater protection than either treatment alone. Salt stress induced proline accumulation, which was further enhanced by the combined application of proline and Si. This increase was associated with elevated transcript levels of key genes involved in proline biosynthesis. Further analyses revealed differential regulation of the glutamate and ornithine pathways contributing to proline biosynthesis under salt stress in <i>M. truncatula</i>. In addition, transcript levels of the silicon transporter gene <i>Lsi2</i> were significantly higher in salt-stressed plants receiving both proline and Si, coinciding with increased Si accumulation in shoots.</p> Conclusions <p>Our findings demonstrate that enhanced proline metabolism and Si accumulation contribute&#xa0;to the improved salt tolerance induced by the exogenous application of proline and Si in <i>M. truncatula</i>.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Mitigation of progressive salt stress in Medicago truncatula by silicon and proline through modulation of proline metabolism and silicon transporter gene expression

  • 期刊论文

  • 发表日期：

    2026年7月29日

查看全文
  • Ahmed El Moukhtari,
  • Séverine Planchais,
  • Cécile Cabassa,
  • Emilie Crilat,
  • Pierre Carol,
  • Mohamed Farissi,
  • Arnould Savouré

摘要

Background and Aims

Salt stress is a major constraint limiting crop productivity worldwide. Among the sustainable and environmentally friendly strategies proposed to mitigate salt-induced reductions in crop growth and yield, the exogenous application of proline and silicon (Si) has attracted increasing attention.

Methods

Here, we investigated the effects of the individual and combined application of 20 mM proline and 1.24 mM Si on Medicago truncatula, a model legume, exposed to salt stress.

Results

The results showed that continuous exposure to 120 mM NaCl significantly impaired plant growth indexes, including shoot and root dry weight, plant height, leaf number and area. Exogenous application of either proline or Si partially alleviated the detrimental effects of NaCl on these growth indexes. Notably, the combined application of proline and Si conferred greater protection than either treatment alone. Salt stress induced proline accumulation, which was further enhanced by the combined application of proline and Si. This increase was associated with elevated transcript levels of key genes involved in proline biosynthesis. Further analyses revealed differential regulation of the glutamate and ornithine pathways contributing to proline biosynthesis under salt stress in M. truncatula. In addition, transcript levels of the silicon transporter gene Lsi2 were significantly higher in salt-stressed plants receiving both proline and Si, coinciding with increased Si accumulation in shoots.

Conclusions

Our findings demonstrate that enhanced proline metabolism and Si accumulation contribute to the improved salt tolerance induced by the exogenous application of proline and Si in M. truncatula.