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Background and Aims <p>Plants face escalating threats from biotic stresses, which severely compromise agricultural productivity. To address this challenge, a novel composite microbial inoculant (<i>Streptomyces</i> sp. SF1 + <i>Bacillus cereus</i> G2) with dual biocontrol and plant growth-promotion capabilities was developed in a previous study.</p> Methods <p>In the present study, the medium composition and fermentation conditions of the SF1 + G2 agent were optimized using single-factor and orthogonal experiments based on bacterial biomass. A pot experiment was executed to assess the effectiveness of the SF1 + G2 agent on the plant (<i>Dolichos lablab</i> L.) and its rhizosphere soil under stresses from pathogenic fungi <i>Rhizoctonia solani</i>, <i>Sclerotinia sclerotiorum</i>,<i> and Fusarium oxysporum.</i></p> Results <p>The process optimization results indicated that glucose (15-25&#xa0;g/L) and yeast extract (10.89–18.15&#xa0;g/L) were the optimal carbon–nitrogen sources in the culture medium. The optimal fermentation conditions were as follows: fermentation time 6&#xa0;days, inoculation interval 1&#xa0;day, inoculation ratio 1:3, inoculation amount 0.5%, initial pH 6.0, and liquid volume 120/250&#xa0;mL. SF1 + G2 agents significantly promoted plant growth and reduced the disease incidence rate in <i>D. lablab</i> seedlings infected by pathogenic fungi, as well as increased available nutrient contents and enzyme activities in rhizosphere soil. Concurrently, it elevated the activity of plant antioxidant enzymes, reduced the accumulation of reactive oxygen species (ROS) and membrane peroxidation, thereby enhancing the plant stress resistance and promoting its growth.</p> Conclusion <p>In summary, the SF1 + G2 agent exhibits significant synergistic effects on biological control and promotion, enhancing the plant's tolerance to fungal pathogens and demonstrating high potential as an eco-friendly biological fertilizer for sustainable agriculture.</p>

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Development of a composite microbial agent beneficial to improve plant growth and inhibit disease occurrence under pathogenic fungus stress

  • 期刊论文

  • 发表日期：

    2026年6月26日

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  • Sixuan Li,
  • Xiangjuan Yu,
  • Duoyong Lang,
  • Tianjiao Jia,
  • Xiaokang Li,
  • Xiaojia Zhang,
  • Xinhui Zhang

摘要

Background and Aims

Plants face escalating threats from biotic stresses, which severely compromise agricultural productivity. To address this challenge, a novel composite microbial inoculant (Streptomyces sp. SF1 + Bacillus cereus G2) with dual biocontrol and plant growth-promotion capabilities was developed in a previous study.

Methods

In the present study, the medium composition and fermentation conditions of the SF1 + G2 agent were optimized using single-factor and orthogonal experiments based on bacterial biomass. A pot experiment was executed to assess the effectiveness of the SF1 + G2 agent on the plant (Dolichos lablab L.) and its rhizosphere soil under stresses from pathogenic fungi Rhizoctonia solani, Sclerotinia sclerotiorum, and Fusarium oxysporum.

Results

The process optimization results indicated that glucose (15-25 g/L) and yeast extract (10.89–18.15 g/L) were the optimal carbon–nitrogen sources in the culture medium. The optimal fermentation conditions were as follows: fermentation time 6 days, inoculation interval 1 day, inoculation ratio 1:3, inoculation amount 0.5%, initial pH 6.0, and liquid volume 120/250 mL. SF1 + G2 agents significantly promoted plant growth and reduced the disease incidence rate in D. lablab seedlings infected by pathogenic fungi, as well as increased available nutrient contents and enzyme activities in rhizosphere soil. Concurrently, it elevated the activity of plant antioxidant enzymes, reduced the accumulation of reactive oxygen species (ROS) and membrane peroxidation, thereby enhancing the plant stress resistance and promoting its growth.

Conclusion

In summary, the SF1 + G2 agent exhibits significant synergistic effects on biological control and promotion, enhancing the plant's tolerance to fungal pathogens and demonstrating high potential as an eco-friendly biological fertilizer for sustainable agriculture.