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Aims <p><i>Phytophthora</i><i> capsici</i>&#xa0;(<i>P. capsici</i>, <i>Pc</i>) is an oomycete pathogen causing pepper blight, while&#xa0;<i>Trichoderma guizhouense</i>&#xa0;(<i>T. guizhouense</i>, <i>Tg</i>) exhibits robust antagonistic activity against this pathogen. Fungi-oomycete antagonism is mainly mediated by secondary metabolites (SMs), however, the types of SMs and their regulatory mechanism remain unclear.</p> Methods <p>By integrating&#xa0;metabolomic and&#xa0;transcriptomic&#xa0;data, combined with genetic editing, this study deciphered the role of transcription factor <i>Tg</i>SclB1 in <i>T. guizhouense</i> during its antagonism against <i>P. capsici</i>.</p> Results <p>Antagonism assays revealed that <i>TgsclB1</i> gene deletion (Δ<i>TgsclB1</i>) diminished the inhibitory efficacy of <i>Tg</i>-derived SMs against <i>Pc</i>, with a 14.6% increase in pepper disease indices compared to wild-type (<i>Tg</i>-WT). Metabolomic analysis identified mono-methyl phthalate (MMP) as a key anti-oomycete compound, where in the OE-<i>TgsclB1</i> (<i>TgsclB1</i> overexpression strain) exhibited 176.0% greater MMP accumulation compared to the <i>Tg</i>-WT. Furthermore, transcriptomic analysis linked <i>Tg</i>SclB1 to the transcriptional activation of <i>TgpdC</i> (encoding pyridoxal-dependent decarboxylase), thereby driving MMP biosynthesis. Pot experiments confirmed that exogenous MMP application and OE-<i>TgpdC</i> (<i>TgpdC</i> overexpression strain) inoculation significantly reduced pepper disease indices. Overall, <i>Tg</i>SclB1 orchestrates oomycete antagonism by regulating <i>TgpdC</i> expression to mediate MMP biosynthesis.</p> Conclusions <p>This study clarified the “<i>Tg</i>SclB1-<i>TgpdC</i>-MMP” regulatory pathway underlying the biocontrol activity of&#xa0;<i>T. guizhouense</i>&#xa0;against oomycetes, providing insights for the construction of biocontrol strains and the development of green control strategies for pepper blight.</p>

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TgSclB1 orchestrates anti-oomycete activity by regulating mono-methyl phthalate biosynthesis in Trichoderma guizhouense

  • 期刊论文

  • 发表日期：

    2026年4月29日

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  • Yang Liu,
  • Xiaoteng Shi,
  • Tuokai Wang,
  • Lvxun Tang,
  • Yihao Zhou,
  • Bozheng Lin,
  • Linhua Cao,
  • Siqiao Chen,
  • Tuo Li,
  • Dongyang Liu,
  • Qirong Shen

摘要

Aims

Phytophthora capsici (P. capsici, Pc) is an oomycete pathogen causing pepper blight, while Trichoderma guizhouense (T. guizhouense, Tg) exhibits robust antagonistic activity against this pathogen. Fungi-oomycete antagonism is mainly mediated by secondary metabolites (SMs), however, the types of SMs and their regulatory mechanism remain unclear.

Methods

By integrating metabolomic and transcriptomic data, combined with genetic editing, this study deciphered the role of transcription factor TgSclB1 in T. guizhouense during its antagonism against P. capsici.

Results

Antagonism assays revealed that TgsclB1 gene deletion (ΔTgsclB1) diminished the inhibitory efficacy of Tg-derived SMs against Pc, with a 14.6% increase in pepper disease indices compared to wild-type (Tg-WT). Metabolomic analysis identified mono-methyl phthalate (MMP) as a key anti-oomycete compound, where in the OE-TgsclB1 (TgsclB1 overexpression strain) exhibited 176.0% greater MMP accumulation compared to the Tg-WT. Furthermore, transcriptomic analysis linked TgSclB1 to the transcriptional activation of TgpdC (encoding pyridoxal-dependent decarboxylase), thereby driving MMP biosynthesis. Pot experiments confirmed that exogenous MMP application and OE-TgpdC (TgpdC overexpression strain) inoculation significantly reduced pepper disease indices. Overall, TgSclB1 orchestrates oomycete antagonism by regulating TgpdC expression to mediate MMP biosynthesis.

Conclusions

This study clarified the “TgSclB1-TgpdC-MMP” regulatory pathway underlying the biocontrol activity of T. guizhouense against oomycetes, providing insights for the construction of biocontrol strains and the development of green control strategies for pepper blight.