Background <p>The price for wheat is driving the expansion of agricultural monocropping practices, which in turn facilitate the spread of phytopathogenic fungal infestation. Inoculating plants/ seeds with beneficial bacteria offers a sustainable alternative to chemical fungicides. Predatory myxobacteria are characterized by their production of various lytic secondary metabolites including fungicides. While their modes of action&#xa0;with prey are well studied, the direct application of myxobacteria in plants remains largely unexplored.</p> Aims <p>The aim of this study was to assess whether seed inoculation with myxobacteria has a general effect on plant growth and can control take-all disease caused by <i>Gaeumannomyces tritici</i> (Ggt) in wheat in vivo.</p> Methods <p>The inhibition potential of five different myxobacteria on ten soil-borne phytopathogenic fungi was examined in vitro. The effective myxobacteria, <i>Haliangium ochraceum</i> and <i>Myxococcus virescens,</i> were used as seed inoculants of winter wheat, which was grown for four weeks in soil infected with the pathogen Ggt in an in vivo greenhouse experiment.</p> Results <p> Myxobacteria inoculation did not impair plant growth (with and without Ggt infection), and <i>M. virescens</i> even showed a slight growth-promoting effect. Furthermore, myxobacteria inoculated plants showed a 50% reduction in symptoms of Ggt infestation compared to non-inoculated controls. Microbial (bacterial and archaeal) community profiling revealed no significant changes in rhizosphere and minor changes in bulk soil in response to myxobacteria inoculation.</p> Conclusions <p>Our study suggests that myxobacteria might be promising candidates for new plant-beneficial inoculants.</p>