Scholarlink

Aims <p>While manure application is crucial for maintaining soil fertility, it may pose a risk to public health by promoting the spread of antibiotic resistance genes (ARGs). Nevertheless, the long-term effects of fertilization practices on the accumulation and dynamics of ARGs remain poorly understood.</p> Methods <p>We employed metagenomic sequencing to investigate changes in soil ARGs and bacterial communities and to identify factors influencing these dynamics in a 35-year field fertilization experiment. Four treatments were included as chemical nitrogen fertilizer (N), manure (M), chemical nitrogen plus manure (NM), and control with no fertilization (CK).</p> Results <p>Both M and NM treatments significantly increased the abundance of ARGs and bacterial communities, while also shifting their profiles in the soils. Strong and significant correlations between ARGs and bacterial communities were observed through network analyses. Dominant bacterial phyla (<i>Proteobacteria</i>, <i>Actinobacteria</i>, <i>Firmicutes</i>, <i>Nitrospirae</i>, <i>Gemmatimonadetes</i>, and <i>Acidobacteria</i>) were identified as ARG hosts. Co-occurrence network analysis and random forest models detected several key ARGs, which were classified into two principal mechanisms: antibiotic efflux (<i>mdtP</i>, <i>mtrC</i>, and <i>mexN</i>) and antibiotic target alteration (<i>PmrB</i>, <i>arnA</i>, <i>aminocoumarin resistant cysB</i>, <i>Sser_parE_FLO</i>, <i>Ngon_parC_FLO</i>). Soil organic carbon and total nitrogen were the dominant soil properties influencing ARG abundance. Furthermore, these soil properties indirectly affected ARG abundance by shaping the abundance of bacterial communities.</p> Conclusions <p>Altogether, our results provide insights into the role of long-term fertilization in regulating ARG distribution in the soils and highlight potential risks associated with the external introduction of manure-derived ARGs from fertilization practices, particularly the potential transfer of resistance genes to plants.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Long-term wheat field fertilization shapes soil microbial antibiotic resistance genes through bacterial community modulation on the Loess Plateau

  • 期刊论文

  • 发表日期：

    2026年3月3日

查看全文
  • Mengmeng Wen,
  • Rong Hu,
  • Jinfa Chen,
  • Caidi Yang,
  • Jun Wang,
  • Yang Liu

摘要

Aims

While manure application is crucial for maintaining soil fertility, it may pose a risk to public health by promoting the spread of antibiotic resistance genes (ARGs). Nevertheless, the long-term effects of fertilization practices on the accumulation and dynamics of ARGs remain poorly understood.

Methods

We employed metagenomic sequencing to investigate changes in soil ARGs and bacterial communities and to identify factors influencing these dynamics in a 35-year field fertilization experiment. Four treatments were included as chemical nitrogen fertilizer (N), manure (M), chemical nitrogen plus manure (NM), and control with no fertilization (CK).

Results

Both M and NM treatments significantly increased the abundance of ARGs and bacterial communities, while also shifting their profiles in the soils. Strong and significant correlations between ARGs and bacterial communities were observed through network analyses. Dominant bacterial phyla (Proteobacteria, Actinobacteria, Firmicutes, Nitrospirae, Gemmatimonadetes, and Acidobacteria) were identified as ARG hosts. Co-occurrence network analysis and random forest models detected several key ARGs, which were classified into two principal mechanisms: antibiotic efflux (mdtP, mtrC, and mexN) and antibiotic target alteration (PmrB, arnA, aminocoumarin resistant cysB, Sser_parE_FLO, Ngon_parC_FLO). Soil organic carbon and total nitrogen were the dominant soil properties influencing ARG abundance. Furthermore, these soil properties indirectly affected ARG abundance by shaping the abundance of bacterial communities.

Conclusions

Altogether, our results provide insights into the role of long-term fertilization in regulating ARG distribution in the soils and highlight potential risks associated with the external introduction of manure-derived ARGs from fertilization practices, particularly the potential transfer of resistance genes to plants.