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Background and aims <p>Drought (D), salt (S), and drought-salt (SD) stresses adversely affect the growth and yield of&#xa0;<i>Glycyrrhiza uralensis</i> (<i>G. uralensis</i>). In our earlier research, silicon (Si) enhances the stress tolerance of <i>G. uralensis</i> by regulating proline and glycyrrhizic acid accumulation—key metabolites in nitrogen (N) and secondary metabolism.</p> Methods <p>Transcriptomic sequencing combined with physiological-biochemical analysis was employed to investigate Si-mediated accumulation of N and secondary metabolites in <i>G. uralensis</i> roots under D and/or S stresses, along with the underlying mechanisms.</p> Results <p>Findings indicate that Si increased proline and soluble protein contents by regulating&#xa0;<i>NR</i>,&#xa0;<i>NiR</i>, and&#xa0;<i>GS</i>&#xa0;gene expression under S and SD stresses. Simultaneously, Si elevated liquiritigenin and liquiritin contents by regulating&#xa0;<i>PAL</i>,&#xa0;<i>C4H</i>,&#xa0;<i>4CL</i>, and&#xa0;<i>CHS</i>&#xa0;gene expression under D and S stresses. Weighted Gene Co-expression Network Analysis (WGCNA) revealed that Si may regulate the expression of transcription factor genes, including <i>MYB</i>, <i>bHLH</i>, and <i>ERF</i>, consequently affecting phenylpropanoid biosynthesis, amino acid metabolism, N metabolism, and flavonoid biosynthesis pathways, ultimately controlling N and secondary metabolism in <i>G. uralensis</i> roots.</p> Conclusions <p>&#xa0;The study elucidated the molecular mechanisms through which Si regulated the accumulation of N and secondary metabolites in <i>G. uralensis</i> roots under D and/or S stresses, providing a theoretical basis for improving its yield and quality under drought and salt stress environment.</p>

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Silicon-mediated remodeling of nitrogen and secondary metabolism enhances drought and salt tolerance in Glycyrrhiza uralensis roots

  • 期刊论文

  • 发表日期：

    2026年2月4日

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  • Yonggan Ji,
  • Yufeng Wang,
  • Wenjin Zhang,
  • Gaochang Cui,
  • Duoyong Lang,
  • Xinhui Zhang

摘要

Background and aims

Drought (D), salt (S), and drought-salt (SD) stresses adversely affect the growth and yield of Glycyrrhiza uralensis (G. uralensis). In our earlier research, silicon (Si) enhances the stress tolerance of G. uralensis by regulating proline and glycyrrhizic acid accumulation—key metabolites in nitrogen (N) and secondary metabolism.

Methods

Transcriptomic sequencing combined with physiological-biochemical analysis was employed to investigate Si-mediated accumulation of N and secondary metabolites in G. uralensis roots under D and/or S stresses, along with the underlying mechanisms.

Results

Findings indicate that Si increased proline and soluble protein contents by regulating NR, NiR, and GS gene expression under S and SD stresses. Simultaneously, Si elevated liquiritigenin and liquiritin contents by regulating PAL, C4H, 4CL, and CHS gene expression under D and S stresses. Weighted Gene Co-expression Network Analysis (WGCNA) revealed that Si may regulate the expression of transcription factor genes, including MYB, bHLH, and ERF, consequently affecting phenylpropanoid biosynthesis, amino acid metabolism, N metabolism, and flavonoid biosynthesis pathways, ultimately controlling N and secondary metabolism in G. uralensis roots.

Conclusions

 The study elucidated the molecular mechanisms through which Si regulated the accumulation of N and secondary metabolites in G. uralensis roots under D and/or S stresses, providing a theoretical basis for improving its yield and quality under drought and salt stress environment.