Scholarlink

Aim <p>In rocky desertification areas, the yield and quality of <i>Juglans sigillata</i> Dode are affected by water shortage caused by seasonal drought, while arbuscular mycorrhizal fungi (AMF) can enhance the drought resistance of plants. The aim was to clarify AMF community structure and demonstrate the role of dominant AMF strains in water uptake and utilization by <i>J. sigillata</i>.</p> Methods <p>AMF community composition and diversity were analyzed using high-throughput amplicon sequencing alongside spore isolation and identification. A controlled two-factor experiment (inoculation with/without&#xa0;<i>Glomus reticulatum</i>; well-watered/drought stress) was conducted on&#xa0;<i>J. sigillata</i>&#xa0;seedlings to verify drought resistance enhancement.</p> Results <p>The diversity survey showed that AMF can form a symbiotic relationship with the roots of <i>J. sigillata</i>. AMF diversity was significantly higher in RD (Rocky desertification) habitats, <i>Glomus</i>&#xa0;(genus) and&#xa0;<i>Glomus reticulatum</i>&#xa0;(species) were the dominant taxa. Correlation analysis revealed that available phosphorus (AP) had the strongest association with the AMF community structure, suggesting it may be a key environmental filter for the AMF community in RD habitats. Results of the pot experiment indicated that under drought stress, inoculation with <i>G. reticulatum</i> alleviated water deficit in plants, promoted biomass accumulation, further enhanced the plants’ photosynthetic capacity and reactive oxygen species (ROS) scavenging capacity, effectively reducing oxidative damage and mitigating the adverse effects of drought on seedlings.</p> Conclusions <p>The rhizosphere soil of&#xa0;<i>J. sigillata</i>&#xa0;harbors diverse AMF resources, dominated by the genus&#xa0;<i>Glomus</i>&#xa0;and specifically&#xa0;<i>G. reticulatum</i>.&#xa0;Inoculation with<i> G. reticulatum</i> can significantly improve the drought resistance of <i>J. sigillata</i> seedlings.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Diversity of AMF in the rhizosphere soil of Juglans sigillata Dode in karst rocky desertification areas and the influence of dominance population Glomus reticulatum on the drought resistance of the seedlings

  • 期刊论文

  • 发表日期：

    2026年1月5日

查看全文
  • Xue Lei,
  • Liqiao Xu,
  • Qing Shang,
  • Dong Huang,
  • Jinyan Chen,
  • Tiantian Fei,
  • Xuejun Pan,
  • Wen’e Zhang

摘要

Aim

In rocky desertification areas, the yield and quality of Juglans sigillata Dode are affected by water shortage caused by seasonal drought, while arbuscular mycorrhizal fungi (AMF) can enhance the drought resistance of plants. The aim was to clarify AMF community structure and demonstrate the role of dominant AMF strains in water uptake and utilization by J. sigillata.

Methods

AMF community composition and diversity were analyzed using high-throughput amplicon sequencing alongside spore isolation and identification. A controlled two-factor experiment (inoculation with/without Glomus reticulatum; well-watered/drought stress) was conducted on J. sigillata seedlings to verify drought resistance enhancement.

Results

The diversity survey showed that AMF can form a symbiotic relationship with the roots of J. sigillata. AMF diversity was significantly higher in RD (Rocky desertification) habitats, Glomus (genus) and Glomus reticulatum (species) were the dominant taxa. Correlation analysis revealed that available phosphorus (AP) had the strongest association with the AMF community structure, suggesting it may be a key environmental filter for the AMF community in RD habitats. Results of the pot experiment indicated that under drought stress, inoculation with G. reticulatum alleviated water deficit in plants, promoted biomass accumulation, further enhanced the plants’ photosynthetic capacity and reactive oxygen species (ROS) scavenging capacity, effectively reducing oxidative damage and mitigating the adverse effects of drought on seedlings.

Conclusions

The rhizosphere soil of J. sigillata harbors diverse AMF resources, dominated by the genus Glomus and specifically G. reticulatum. Inoculation with G. reticulatum can significantly improve the drought resistance of J. sigillata seedlings.