Background and Aims <p>This study investigates how soil terpenoid content and emissions vary with soil depth and between two sites with contrasting tree species in a mixed temperate forest. We aimed to understand how species-specific litter composition and depth-dependent soil conditions shape soil terpenoid content and emissions.</p> Methods <p>Foliar and soil samples were collected from evergreen Douglas fir and deciduous European beech plots under comparable climatic and edaphic conditions. Terpenoid contents in foliage were analyzed, as well as soil terpenoid content and emissions across different soil depths. Additionally, soil CO₂ emissions, δ¹³C values, and physicochemical properties were assessed.</p> Results <p>Douglas fir plots showed substantially higher terpenoid content and emissions than European beech plots, consistent with differences in foliar terpenoid composition. Terpenoids were concentrated in surface layers and declined with depth. Emissions were limited to surface soil in the Douglas fir plot, whereas the European beech plot showed little variation with depth. CO₂ emissions and δ¹³C values varied with depth, indicating a shift in microbial activity and substrate origin from plant-derived litter in surface soils to more microbially processed sources in deeper layers.</p> Conclusions <p>This study indicates that tree species are the primary driver of soil terpenoid dynamics, as species-specific litter chemistry governs both the magnitude and vertical distribution of soil terpenoid content and emissions. Depth-related patterns appear to be associated with microbial activity, the origin of decomposed substrates, and soil physicochemical properties. These findings underscore the importance of accounting for both vegetation composition and depth-resolved soil processes when assessing VOC budgets in forest ecosystems.</p>