Scholarlink

Aims <p>This study aims to elucidate whether native plants (<i>Carex breviculmis</i>, <i>Buddleja davidii</i>, <i>Artemisia annua</i>) can mediate rhizosphere nutrient enrichment that in turn shapes microbial carbon cycling gene expression in a Pb–Zn waste heap.</p> Methods <p>Rhizosphere soil from colonizing plants were collected and analyzed for nutrient status and C-cycling functional genes via metagenomics.</p> Results <p>Native plants significantly enhanced the diversity and restructured C-cycling genes, with <i>A. annua</i> and <i>B. davidii</i> exerting strongest effects. Plant growth, particularly of <i>A. annua</i> and <i>B. davidii</i>, enhanced rhizosphere nutrient content (soil organic carbon, total nitrogen, ammonium nitrogen, and nitrate nitrogen), thereby elevating resource availability. This resource reconfiguration directly drove microbial community restructuring and their adaptive metabolic strategies: microbes preferentially upregulated the relative abundance of C decomposition-related genes (e.g., Glycoside Hydrolases) while downregulating C biosynthesis-related genes (e.g., Glycosyl Transferases). Consequently, the microbial community transitioned from an oligotrophic-adapted state dominated by taxa reliant on recalcitrant organic matter (e.g., <i>Kitasatosporales</i>) to a copiotrophic-responsive state enriched with taxa favoring labile carbon sources (e.g., <i>Sphingomonadales</i> and <i>Solirubrobacterales</i>). Notably, certain Actinobacteria taxa (e.g., <i>Pseudonocardiales</i> and <i>Micromonosporales</i>) exhibited functional flexibility, maintaining capabilities for degrading complex organic substrates while adaptively responding to rhizosphere resource inputs. This microbial shift helps accelerate organic matter turnover and improve nutrient availability in the metal-contaminated soil.</p> Conclusion <p>These findings highlight the critical role of plant–microbe interactions in regulating soil carbon cycling and functional resilience in heavy metal-polluted environments, offering a scientific foundation for developing targeted phytoremediation approaches and improving the efficiency of ecological restoration in metal-contaminated areas.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Plant-induced nutrient enrichment alters microbial carbon cycling gene expression in Pb–Zn contaminated rhizospheres

  • 期刊论文

  • 发表日期：

    2025年8月16日

查看全文
  • Caili Sun,
  • Yongwei Liu,
  • Yajuan Yao,
  • Xiaoyu Lu

摘要

Aims

This study aims to elucidate whether native plants (Carex breviculmis, Buddleja davidii, Artemisia annua) can mediate rhizosphere nutrient enrichment that in turn shapes microbial carbon cycling gene expression in a Pb–Zn waste heap.

Methods

Rhizosphere soil from colonizing plants were collected and analyzed for nutrient status and C-cycling functional genes via metagenomics.

Results

Native plants significantly enhanced the diversity and restructured C-cycling genes, with A. annua and B. davidii exerting strongest effects. Plant growth, particularly of A. annua and B. davidii, enhanced rhizosphere nutrient content (soil organic carbon, total nitrogen, ammonium nitrogen, and nitrate nitrogen), thereby elevating resource availability. This resource reconfiguration directly drove microbial community restructuring and their adaptive metabolic strategies: microbes preferentially upregulated the relative abundance of C decomposition-related genes (e.g., Glycoside Hydrolases) while downregulating C biosynthesis-related genes (e.g., Glycosyl Transferases). Consequently, the microbial community transitioned from an oligotrophic-adapted state dominated by taxa reliant on recalcitrant organic matter (e.g., Kitasatosporales) to a copiotrophic-responsive state enriched with taxa favoring labile carbon sources (e.g., Sphingomonadales and Solirubrobacterales). Notably, certain Actinobacteria taxa (e.g., Pseudonocardiales and Micromonosporales) exhibited functional flexibility, maintaining capabilities for degrading complex organic substrates while adaptively responding to rhizosphere resource inputs. This microbial shift helps accelerate organic matter turnover and improve nutrient availability in the metal-contaminated soil.

Conclusion

These findings highlight the critical role of plant–microbe interactions in regulating soil carbon cycling and functional resilience in heavy metal-polluted environments, offering a scientific foundation for developing targeted phytoremediation approaches and improving the efficiency of ecological restoration in metal-contaminated areas.