Scholarlink

Background and Aims <p>Arsenic (As) is a toxic metalloid element. Rice typically accumulates higher As concentrations than other cereal crops. However, gaps remain in understanding its regulatory networks and tissue-specific accumulation mechanisms.</p> Methods <p>As concentrations in multiple tissues of 533 rice germplasms were determined to identify varieties with stable differences. A total of 270 F<sub>2</sub> individuals, derived from a cross between C159 (low-As variety) × W140 (high-As variety) were used to identify quantitative trait loci (QTLs) associated with As accumulation through inclusive composite interval mapping (ICIM). Subsequently, progeny testing of As concentrations in grain and straw of seven recombinant F<sub>3</sub> lines was conducted to refine the QTL boundaries.</p> Results <p>Correlation analysis revealed a significantly negative correlation between As concentrations and heading date, plant height, and biomass across 533 rice germplasms. Five QTLs associated with As concentration were identified, including a major QTL (<i>qACSM-1–2</i>) localized on chromosome 1L. This locus explained 16.51%, 9.29%, and 7.32% of the phenotypic variation in As concentrations in straw at the maturity stage, grain at the maturity stage and root at the seedling stage, respectively. Fine-mapping delimited the candidate region of <i>qACSM-1–2</i> to a 381&#xa0;kb interval flanked by markers 1RM40290 and 1RM40671, within which six candidate genes were prioritized. Comparisons with previous studies suggest that <i>qACSM-1–2</i> is potentially novel.</p> Conclusions <p>A novel QTL <i>qACSM-1–2</i> was identified for regulating rice straw and grain As concentrations under field conditions. These findings support the use of marker-assisted selection (MAS) to incorporate this locus into elite rice cultivars.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Identification of QTLs and validation of qACSM-1–2 associated with arsenic concentrations in rice (Oryza sativa L.)

  • 期刊论文

  • 发表日期：

    2025年8月15日

查看全文
  • Fugang Huang,
  • Kangshun Huang,
  • Jinhao Bao,
  • Lei Huang,
  • Lingyue Zhang,
  • Pengyuan Guo,
  • Zongyue Jiang,
  • Fangchi Wei,
  • Mengfan Liu,
  • Zihan Zhao,
  • Sihao Yang,
  • Xingming Lian,
  • Meng Yang

摘要

Background and Aims

Arsenic (As) is a toxic metalloid element. Rice typically accumulates higher As concentrations than other cereal crops. However, gaps remain in understanding its regulatory networks and tissue-specific accumulation mechanisms.

Methods

As concentrations in multiple tissues of 533 rice germplasms were determined to identify varieties with stable differences. A total of 270 F2 individuals, derived from a cross between C159 (low-As variety) × W140 (high-As variety) were used to identify quantitative trait loci (QTLs) associated with As accumulation through inclusive composite interval mapping (ICIM). Subsequently, progeny testing of As concentrations in grain and straw of seven recombinant F3 lines was conducted to refine the QTL boundaries.

Results

Correlation analysis revealed a significantly negative correlation between As concentrations and heading date, plant height, and biomass across 533 rice germplasms. Five QTLs associated with As concentration were identified, including a major QTL (qACSM-1–2) localized on chromosome 1L. This locus explained 16.51%, 9.29%, and 7.32% of the phenotypic variation in As concentrations in straw at the maturity stage, grain at the maturity stage and root at the seedling stage, respectively. Fine-mapping delimited the candidate region of qACSM-1–2 to a 381 kb interval flanked by markers 1RM40290 and 1RM40671, within which six candidate genes were prioritized. Comparisons with previous studies suggest that qACSM-1–2 is potentially novel.

Conclusions

A novel QTL qACSM-1–2 was identified for regulating rice straw and grain As concentrations under field conditions. These findings support the use of marker-assisted selection (MAS) to incorporate this locus into elite rice cultivars.