Background and aims Achnatherum inebrians is main poisonous bunchgrass in the grassland of northern China. Nematodes are the most prevalent and diverse group of multicellular animals in terrestrial ecosystems. The effects of endophytic fungus – A. inebrians symbiosis on nematodes were investigate to find a solution to control harmful nematodes in the soil so the crop can reach high yield level. Methods Field experiments were conducted to investigate the effects of endophytic fungi on soil nematode communities in the rhizosphere of A. inebrians, and the effects of leaf and seed extract and root exudates of endophytic fungus – A. inebrians symbiosis on the hatching of nematodes cysts were measured in laboratory. Results The diversity index of soil nematodes in the roots of A. inebrians was not significantly altered by endophytic fungi. However, grass-endophytic fungi altered the composition of the nematode community in the rhizosphere soil of A. inebrians. In addition, Epichloë endophytes had significant difference on the composition of functional groups of soil nematodes, especially on plant-parasitic nematodes. Nematode community in rhizosphere soil of endophyte-infected (E +) A. inebrians had higher MI, SI, CI and lower PPI, PPI/MI and EI indices. The individual density of predators/omnivores nematode in rhizosphere soil of endophyte-infected (E +) A. inebrians was significantly higher than that of endophyte-free (E-) A. inebrians. With the increase of concentration of stem, seed extract, and root exudates of A. inebrians, the inhibitory effects on hatching of nematode cysts were enhanced. At low concentrations of steam and seed extract as well as root exudates, Epichloë endophytes of A. inebrians affected the hatching effects on H. glycines oocysts and M. incognita cysts masses. Conclusions Endophytic fungi affect the species composition and diversity of nematode communities in the rhizosphere soil of A. inebrians. The extract of stem and seed, and root exudates of symbiont of A. inebrians—epichloë endophytes have great inhibitory effect on hatching of H. glycines oocysts and M. incognita cysts.

The effect of endophyte-infected Achnatherum inebrians on nematode community and the hatching of Heterodera glycines oocysts and Meloidogyne incongnita cysts

期刊论文
发表日期：

2025年4月4日

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Xuekai Wei,
Zheng Liang,
Changhui Guo,
Tingting Ding,
Xiuzhang Li,
Kamran Malik,
Chunjie Li

摘要

Background and aims

Achnatherum inebrians is main poisonous bunchgrass in the grassland of northern China. Nematodes are the most prevalent and diverse group of multicellular animals in terrestrial ecosystems. The effects of endophytic fungus – A. inebrians symbiosis on nematodes were investigate to find a solution to control harmful nematodes in the soil so the crop can reach high yield level.

Methods

Field experiments were conducted to investigate the effects of endophytic fungi on soil nematode communities in the rhizosphere of A. inebrians, and the effects of leaf and seed extract and root exudates of endophytic fungus – A. inebrians symbiosis on the hatching of nematodes cysts were measured in laboratory.

Results

The diversity index of soil nematodes in the roots of A. inebrians was not significantly altered by endophytic fungi. However, grass-endophytic fungi altered the composition of the nematode community in the rhizosphere soil of A. inebrians. In addition, Epichloë endophytes had significant difference on the composition of functional groups of soil nematodes, especially on plant-parasitic nematodes. Nematode community in rhizosphere soil of endophyte-infected (E +) A. inebrians had higher MI, SI, CI and lower PPI, PPI/MI and EI indices. The individual density of predators/omnivores nematode in rhizosphere soil of endophyte-infected (E +) A. inebrians was significantly higher than that of endophyte-free (E-) A. inebrians. With the increase of concentration of stem, seed extract, and root exudates of A. inebrians, the inhibitory effects on hatching of nematode cysts were enhanced. At low concentrations of steam and seed extract as well as root exudates, Epichloë endophytes of A. inebrians affected the hatching effects on H. glycines oocysts and M. incognita cysts masses.

Conclusions

Endophytic fungi affect the species composition and diversity of nematode communities in the rhizosphere soil of A. inebrians. The extract of stem and seed, and root exudates of symbiont of A. inebrians—epichloë endophytes have great inhibitory effect on hatching of H. glycines oocysts and M. incognita cysts.