Scholarlink

Aims <p>This study aimed to investigate the specific effects of native plants on soil microbial diversity and functional gene expression in Pb–Zn slag environments. Bacterial biomarkers were analyzed to address the challenge posed by the vast diversity within soil bacterial communities, which can obscure the contributions of key species to soil nutrient cycling.</p> Methods <p>We utilized 16S rRNA gene sequencing and multivariate statistical analysis to examine the influence of three herbaceous plants—<i>Artemisia annua</i>, <i>Buddleja davidii</i>, and <i>Carex breviculmis</i>—on rhizosphere bacterial biomarkers and the overall bacterial community structure.</p> Results <p>The presence of these plants significantly improved soil nutrient levels and enhanced microbial metabolic functions compared to non-vegetated soils (CK). Specifically, <i>A. annua</i> and <i>B. davidii</i> increased the abundance of genes associated with nitrogen (N) and phosphorus (P) cycling, such as the <i>nar</i> and <i>nir</i> gene families involved in N cycling and the <i>ugp</i> gene family involved in P transport. All three plants increased the abundance of genes (<i>czcABD</i>) encoding ABC transporter proteins, with <i>C. breviculmis</i> showing the highest abundance of metal resistance genes, including <i>ZnuB</i> and <i>ZnuC</i>. Key biomarkers, such as <i>Bradyrhizobium</i>, <i>Bryobacter</i>, and <i>Rokubacteriales</i>, played critical roles. <i>Bradyrhizobium</i> was linked to nitrogen fixation in the rhizosphere of <i>A. annua</i> and <i>B. davidii</i>, while <i>Bryobacter</i> and <i>Rokubacteriales</i> contributed to carbon cycling in the rhizosphere of <i>C. breviculmis</i>. Network analysis demonstrated that plant species significantly reshaped microbial interaction networks, fostering more efficient microbial interactions within the rhizosphere.</p> Conclusions <p>These findings highlight that the enhancement of soil nutrient cycling is primarily driven by increased biomarker abundance and diversity, emphasizing the critical role of bacterial biomarkers in improving soil nutrients in metal-contaminated environments.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

The role of plant-specific rhizosphere bacterial biomarkers in enhancing soil nutrient cycling in Pb–Zn waste slag

  • 期刊论文

  • 发表日期：

    2025年3月10日

查看全文
  • Xiaoyu Lu,
  • Yongwei Liu,
  • Guangneng Zeng,
  • Caili Sun

摘要

Aims

This study aimed to investigate the specific effects of native plants on soil microbial diversity and functional gene expression in Pb–Zn slag environments. Bacterial biomarkers were analyzed to address the challenge posed by the vast diversity within soil bacterial communities, which can obscure the contributions of key species to soil nutrient cycling.

Methods

We utilized 16S rRNA gene sequencing and multivariate statistical analysis to examine the influence of three herbaceous plants—Artemisia annua, Buddleja davidii, and Carex breviculmis—on rhizosphere bacterial biomarkers and the overall bacterial community structure.

Results

The presence of these plants significantly improved soil nutrient levels and enhanced microbial metabolic functions compared to non-vegetated soils (CK). Specifically, A. annua and B. davidii increased the abundance of genes associated with nitrogen (N) and phosphorus (P) cycling, such as the nar and nir gene families involved in N cycling and the ugp gene family involved in P transport. All three plants increased the abundance of genes (czcABD) encoding ABC transporter proteins, with C. breviculmis showing the highest abundance of metal resistance genes, including ZnuB and ZnuC. Key biomarkers, such as Bradyrhizobium, Bryobacter, and Rokubacteriales, played critical roles. Bradyrhizobium was linked to nitrogen fixation in the rhizosphere of A. annua and B. davidii, while Bryobacter and Rokubacteriales contributed to carbon cycling in the rhizosphere of C. breviculmis. Network analysis demonstrated that plant species significantly reshaped microbial interaction networks, fostering more efficient microbial interactions within the rhizosphere.

Conclusions

These findings highlight that the enhancement of soil nutrient cycling is primarily driven by increased biomarker abundance and diversity, emphasizing the critical role of bacterial biomarkers in improving soil nutrients in metal-contaminated environments.