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<p>A method for quantitative determination of the sophoricoside (genistein 4′-<i>O</i>-′-D-glucopyranoside) content in Japanese sophora (<i>Sophora japonica</i> L.) fruit by reversed-phase HPLC in gradient mode on a Milichrom-6 microcolumn liquid chromatograph using a reference standard of sophoricoside isolated from the studied raw material using column chromatography and identified using UV, PMR, and <sup>13</sup>C NMR spectroscopy has been developed. It was determined that the sophoricoside content in Sophora japonica fruit varied from 5.84 ± 0.22 to 6.67 ± 0.25%. The error of a single determination of the sophoricoside content in <i>Sophora japonica</i> fruit was ±4.51% with a confidence probability of 95%.</p>

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HPLC Determination of Sophoricoside Content in Japanese Sophora Fruit

  • 期刊论文

  • 发表日期：

    2025年11月1日

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  • M. K. Cherednik,
  • V. A. Kurkin,
  • A. A. Andreev

摘要

A method for quantitative determination of the sophoricoside (genistein 4′-O-′-D-glucopyranoside) content in Japanese sophora (Sophora japonica L.) fruit by reversed-phase HPLC in gradient mode on a Milichrom-6 microcolumn liquid chromatograph using a reference standard of sophoricoside isolated from the studied raw material using column chromatography and identified using UV, PMR, and 13C NMR spectroscopy has been developed. It was determined that the sophoricoside content in Sophora japonica fruit varied from 5.84 ± 0.22 to 6.67 ± 0.25%. The error of a single determination of the sophoricoside content in Sophora japonica fruit was ±4.51% with a confidence probability of 95%.