Scholarlink

<p>Aquantitative determination method for sulfasalazine in rabbit blood plasma and Caco-2 cell culture medium by high-performance liquid chromatography with tandem mass spectrometric detection has been developed. Methanol with valsartan added as an internal standard was used for sample preparation. The method was validated for the parameters selectivity, limit of quantitation, linearity, intra- and inter-run accuracy and precision, sample carryover, matrix effect, extraction effect, and sample stability. The confirmed analytical range of the method was 5 – 5,000 ng/mL for cell culture medium and 50 – 20,000 ng/mL for rabbit blood plasma. The method could be used to study the pharmacokinetics of sulfasalazine in rabbits and to assess the activity of breast cancer resistance protein (BCRP) in experiments <i>in vitro</i> and <i>in vivo</i>, the substrate of which was sulfasalazine.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Development and Validation of a Quantitative Determination Method for Sulfasalazine in Rabbit Blood Plasma and Cell Culture Medium by HPLC-MS/MS

  • 期刊论文

  • 发表日期：

    2025年6月1日

查看全文
  • M. I. Povetko,
  • P. Yu. Mylnikov,
  • Yu. Tranova,
  • A. V. Shchulkin,
  • A. S. Polupanov,
  • E. N. Yakusheva

摘要

Aquantitative determination method for sulfasalazine in rabbit blood plasma and Caco-2 cell culture medium by high-performance liquid chromatography with tandem mass spectrometric detection has been developed. Methanol with valsartan added as an internal standard was used for sample preparation. The method was validated for the parameters selectivity, limit of quantitation, linearity, intra- and inter-run accuracy and precision, sample carryover, matrix effect, extraction effect, and sample stability. The confirmed analytical range of the method was 5 – 5,000 ng/mL for cell culture medium and 50 – 20,000 ng/mL for rabbit blood plasma. The method could be used to study the pharmacokinetics of sulfasalazine in rabbits and to assess the activity of breast cancer resistance protein (BCRP) in experiments in vitro and in vivo, the substrate of which was sulfasalazine.