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<p>Plasma-activated solution (PAS), as an application of low-temperature atmospheric plasma, has received extensive attention due to the remarkable ability to inactivate cancer cells. Nevertheless, the preponderance of prior research has predominantly concentrated on the direct therapeutic intervention targeting cancer cells, thereby inadvertently neglecting the investigation into their inactivation processes within the complex human physiological microenvironment. In this paper, MCF-7 and human umbilical vein endothelial cells (HUVEC) are utilized in suppressed proliferation‌ experiments by PAS. The cell viability of MCF-7 was found to be 23% when 100 µL PAS treated the cells for 10&#xa0;s alone. Simultaneously, it was found that the cell viability of HUVEC cells treated with PAS was 60% under the same experimental conditions. This indicated that HUVEC cells have a good resistance to PAS. Furthermore, culture medium(CM) was applied in this study to simulate the human internal environment in order to investigate the impact of the human internal environment on the inactivation of MCF-7 cells. The cell viability of MCF-7 cells is found to be 82% by 100 µL PAS at 110&#xa0;s with in simulated human internal environment. Researches demonstrated that cysteine, methionine, phenylalanine, tyrosine, tryptophan, arginine and glucose in the CM can play a major role in the reduction of cells suppressed proliferation‌ efficacy, which is likely attributable to the presence of unstable functional groups within them. Approach of Increasing the dose of PAS to 450 µL resulted in the MCF-7 cell viability of 29% for 10&#xa0;s treatment even though the human body environment suppresses the inactivation cancer cells by PAS. This research is anticipated to provide a reference for the inactivation of cancer cells by PAS in vivo.</p>

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Inactivation of MCF-7 Cells by Plasma-Activated Solution Under Simulated Extracellular Fluid

  • 期刊论文

  • 发表日期：

    2026年1月3日

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  • Ruixuan Hui,
  • Jiaxin Li,
  • Meng Zhang,
  • Guoqiang Liu,
  • Tao Zhang,
  • Dongping Liu,
  • Na Lu

摘要

Plasma-activated solution (PAS), as an application of low-temperature atmospheric plasma, has received extensive attention due to the remarkable ability to inactivate cancer cells. Nevertheless, the preponderance of prior research has predominantly concentrated on the direct therapeutic intervention targeting cancer cells, thereby inadvertently neglecting the investigation into their inactivation processes within the complex human physiological microenvironment. In this paper, MCF-7 and human umbilical vein endothelial cells (HUVEC) are utilized in suppressed proliferation‌ experiments by PAS. The cell viability of MCF-7 was found to be 23% when 100 µL PAS treated the cells for 10 s alone. Simultaneously, it was found that the cell viability of HUVEC cells treated with PAS was 60% under the same experimental conditions. This indicated that HUVEC cells have a good resistance to PAS. Furthermore, culture medium(CM) was applied in this study to simulate the human internal environment in order to investigate the impact of the human internal environment on the inactivation of MCF-7 cells. The cell viability of MCF-7 cells is found to be 82% by 100 µL PAS at 110 s with in simulated human internal environment. Researches demonstrated that cysteine, methionine, phenylalanine, tyrosine, tryptophan, arginine and glucose in the CM can play a major role in the reduction of cells suppressed proliferation‌ efficacy, which is likely attributable to the presence of unstable functional groups within them. Approach of Increasing the dose of PAS to 450 µL resulted in the MCF-7 cell viability of 29% for 10 s treatment even though the human body environment suppresses the inactivation cancer cells by PAS. This research is anticipated to provide a reference for the inactivation of cancer cells by PAS in vivo.