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<p>Spoiled, fermented, and pickled foods, along with stale beer and wine, often contain elevated levels of tyramine, which may not be effectively metabolized in the intestine or liver. Accumulation of excess tyramine can trigger hypertensive crises and dangerously high blood pressure. To address this, fiber optic probes coated with TiO₂ nanoparticles, along with additional layers of enzyme and 5,10,15,20-tetrakis(4-carboxyphenyl) porphyrin (TCPP), have been employed for tyramine detection in wine samples. The probes were characterized for their surface topology, morphology, and chemical composition using FESEM, EDX, and NMR techniques. Key performance metrics—such as sensitivity, full width at half maximum (FWHM), figure of merit (FOM), limit of detection (LOD), and limit of quantification (LOQ) were evaluated for both enzyme- and TCPP-based probes. Among them, the enzyme-coated probe demonstrated superior sensitivity and favorable sensing characteristics. Additionally, the sensor exhibited high selectivity and a rapid response time of just a few seconds.</p>

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Enzyme and TCPP based fiber optic probes for tyramine detection in wine

  • 期刊论文

  • 发表日期：

    2025年7月18日

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  • Jyoti,
  • R. K. Verma

摘要

Spoiled, fermented, and pickled foods, along with stale beer and wine, often contain elevated levels of tyramine, which may not be effectively metabolized in the intestine or liver. Accumulation of excess tyramine can trigger hypertensive crises and dangerously high blood pressure. To address this, fiber optic probes coated with TiO₂ nanoparticles, along with additional layers of enzyme and 5,10,15,20-tetrakis(4-carboxyphenyl) porphyrin (TCPP), have been employed for tyramine detection in wine samples. The probes were characterized for their surface topology, morphology, and chemical composition using FESEM, EDX, and NMR techniques. Key performance metrics—such as sensitivity, full width at half maximum (FWHM), figure of merit (FOM), limit of detection (LOD), and limit of quantification (LOQ) were evaluated for both enzyme- and TCPP-based probes. Among them, the enzyme-coated probe demonstrated superior sensitivity and favorable sensing characteristics. Additionally, the sensor exhibited high selectivity and a rapid response time of just a few seconds.