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<p><i>Dalbergia sissoo</i> (Shisham), a multipurpose pioneer timber species native to South Asia, is a significantly important tree species both economically and ecologically. This study aims to develop a reliable and efficient in vitro clonal propagation protocol for <i>D. sissoo</i> using nodal segments from ten genotypes. Plant growth regulators (PGRs) and genotypes both considerably affected the rate of in vitro multiplication and rooting percentage, while PGR treatments also significantly affected the bud induction response. For multiple shoot induction, three PGRs—BAP, KIN, and NAA were evaluated separately or in combination. Highest shoot induction (78%) was obtained in combination of BAP (4.44&#xa0;µM) with NAA (2.69&#xa0;µM), whereas the most optimal shoot multiplication rate (2.41) was obtained in MS medium supplemented with BAP (4.44&#xa0;µM) plus NAA (1.34&#xa0;µM). Genotype DS-14 had maximum rate of multiplication (2.24). Amongst the three PGRs (IBA, IAA and NAA) tested for rhizogenesis of the elongated shoots, IBA (4.92&#xa0;µM) was found superior for root induction (61%). The results were significant at <i>p</i> &lt; 0.05. Fidelity test of in vitro plantlets using twelve start codon targeted markers revealed that regenerated plantlets from ten genotypes were true to type with their respective mother plants. This efficient and reproducible in vitro regeneration protocol for <i>D. sissoo</i> can be utilized for the clonal propagation of elite genotypes, as there is a high demand for <i>D. sissoo</i> in clonal forestry for its timber due to its good adaptability in plantation and agroforestry sectors.</p>

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Genotypic influence on in vitro propagation and genetic stability of Dalbergia sissoo

  • 期刊论文

  • 发表日期：

    2025年7月29日

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  • Monika Chauhan,
  • Yashaswi Singh,
  • Parminder Singh,
  • Komal Rani,
  • Shambhavi Yadav,
  • Ajay Thakur

摘要

Dalbergia sissoo (Shisham), a multipurpose pioneer timber species native to South Asia, is a significantly important tree species both economically and ecologically. This study aims to develop a reliable and efficient in vitro clonal propagation protocol for D. sissoo using nodal segments from ten genotypes. Plant growth regulators (PGRs) and genotypes both considerably affected the rate of in vitro multiplication and rooting percentage, while PGR treatments also significantly affected the bud induction response. For multiple shoot induction, three PGRs—BAP, KIN, and NAA were evaluated separately or in combination. Highest shoot induction (78%) was obtained in combination of BAP (4.44 µM) with NAA (2.69 µM), whereas the most optimal shoot multiplication rate (2.41) was obtained in MS medium supplemented with BAP (4.44 µM) plus NAA (1.34 µM). Genotype DS-14 had maximum rate of multiplication (2.24). Amongst the three PGRs (IBA, IAA and NAA) tested for rhizogenesis of the elongated shoots, IBA (4.92 µM) was found superior for root induction (61%). The results were significant at p < 0.05. Fidelity test of in vitro plantlets using twelve start codon targeted markers revealed that regenerated plantlets from ten genotypes were true to type with their respective mother plants. This efficient and reproducible in vitro regeneration protocol for D. sissoo can be utilized for the clonal propagation of elite genotypes, as there is a high demand for D. sissoo in clonal forestry for its timber due to its good adaptability in plantation and agroforestry sectors.