<p>The development of preventive and therapeutic methods based on the principles of hypoxic conditioning is a pressing issue for experimental research, given the high efficacy of such interventions in the prevention and correction of a wide range of pathological conditions. We have previously developed hypoxic conditioning regimens using moderate periodic normobaric hypoxia (PNH) created by inhaling gas mixtures with reduced oxygen content. The aim of the present study was to investigate the behavioral effects of the PNH regimens developed in our work and applied independently rather than as conditioning for pathological states in rats. No significant differences in behavioral measures of rats subjected to PNH as compared with control animals were found in the open field, elevated plus maze, or novel object recognition tests. Thus, the PNH regimens used here do not have intrinsic effects on behavior and can be used for conditioning without the risk of side effects.</p>