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<p>Preeclampsia (PE) could cause neurodevelopmental disorders in offspring, primarily associated with alterations in pro-angiogenic factors. Our objective was to determine the expression levels of VEGF, PGF, pY951-VEGFR2, and pY1175-VEGFR2 in the hippocampus of offspring of preeclamptic mothers and to evaluate the effect of maternal PE on spatial working memory in the offspring. For this, ten Sprague–Dawley pregnant rats were divided into control group (CON; <i>n</i> = 5) and PE group induced by L-NAME (PE; <i>n</i> = 5). After delivery, the offspring were weaning on postnatal day 21 (P21) and categorized: males from CON mothers (M-CON, <i>n</i>:6) and PE mothers (M-PE, <i>n</i> = 6); females from CON mothers (F-CON, <i>n</i> = 6) and PE mothers (F-PE, <i>n</i> = 6). Spatial working memory was evaluated (Y-maze), and hippocampal tissue was subsequently extracted for the analysis of VEGF, PGF, pY951-VEGFR2, and pY1175-VEGFR2 levels by western blot. Our results indicate that VEGF and PGF levels decrease in offspring from PE mothers. pY1175-VEGFR2 showed no differences, and pY951-VEGFR2 was overexpressed in M-PE. In conclusion, PE decreases PGF and VEGF in the hippocampus of the offspring, and its effects on VEGFR2 are dependent on the phosphorylation site and the sex. These findings may help explain the reduced spatial working memory observed in offspring of preeclamptic mothers.</p>

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Effects of preeclampsia on the hippocampus of offspring in rats

  • 期刊论文

  • 发表日期：

    2025年6月1日

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  • Martina Valencia-Narbona,
  • Claudio Ortiz-Soriano,
  • Agustín Dotte-González,
  • Isabel Cuevas-Quezada,
  • Nicolás Acuña-Broerse,
  • Álvaro Riquelme-Zuñiga,
  • Iván Riveros-López,
  • Michael Zamora-Covarrubias,
  • Andrea González-Rojas

摘要

Preeclampsia (PE) could cause neurodevelopmental disorders in offspring, primarily associated with alterations in pro-angiogenic factors. Our objective was to determine the expression levels of VEGF, PGF, pY951-VEGFR2, and pY1175-VEGFR2 in the hippocampus of offspring of preeclamptic mothers and to evaluate the effect of maternal PE on spatial working memory in the offspring. For this, ten Sprague–Dawley pregnant rats were divided into control group (CON; n = 5) and PE group induced by L-NAME (PE; n = 5). After delivery, the offspring were weaning on postnatal day 21 (P21) and categorized: males from CON mothers (M-CON, n:6) and PE mothers (M-PE, n = 6); females from CON mothers (F-CON, n = 6) and PE mothers (F-PE, n = 6). Spatial working memory was evaluated (Y-maze), and hippocampal tissue was subsequently extracted for the analysis of VEGF, PGF, pY951-VEGFR2, and pY1175-VEGFR2 levels by western blot. Our results indicate that VEGF and PGF levels decrease in offspring from PE mothers. pY1175-VEGFR2 showed no differences, and pY951-VEGFR2 was overexpressed in M-PE. In conclusion, PE decreases PGF and VEGF in the hippocampus of the offspring, and its effects on VEGFR2 are dependent on the phosphorylation site and the sex. These findings may help explain the reduced spatial working memory observed in offspring of preeclamptic mothers.