<p>A significant issue in the rehabilitation of movement disorders using neural interfaces is that of the muscle activity supporting implementation of motor imagery. Published data on this question are contradictory. We report here an analysis of the EMG activity of the lower leg and thigh muscles of 40 healthy volunteers working with a neural interface based on kinesthetic imagination of walking on the spot and supplemented with a “Biokin” robotic limb movement device (mechanotherapy), which is activated in response to successful motor imagery. On average for all participants in the experiment, working with the neural interface led to an increase in muscle activity during walking motor imagery as compared with the state of rest, while mechanotrainer activation (MTA) produced an additional increase in muscle activity, its effect being more pronounced in the muscles of the leg with which walking motor imagery started. The nature of muscle reactions to the walking motor imagery task was found to be individual. With MTA and working with a neural interface, the number of experimental participants with marked EMG activity increased, as did the number of significant correlations between the activity of the muscles of the lower limbs. Thus, the use of neural interfaces based on walking motor imagery and MTA as feedback led to activation of the lower limb muscles, which is important in clinical practice during movement rehabilitation.</p>