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<p>Periodontitis is a highly prevalent chronic oral inflammatory disease. It is featured by sustained inflammation, progressive periodontal tissue damage, and irreversible alveolar bone resorption, which remains a critical clinical challenge. In this study, a core–shell composite silk fibroin nanoparticle delivery system loaded with doxycycline (DOX) was successfully constructed. Firstly, ethanol-induced modification combined with silk fibroin self-assembly technology was applied to fabricate DOX-loaded silk fibroin microspheres. Based on electrostatic adsorption, layer-by-layer (LBL) self-assembly was further adopted to alternately adsorb chitosan (CS)-DOX and silk fibroin on the microsphere surface, thereby obtaining core–shell structured composite nanoparticles with a drug-loaded inner core. The as-prepared nanocarrier achieves favorable sustained drug release, antibacterial activity, and osteogenic potency. In vitro and in vivo experiments verified that the synthesized nanoparticles exhibit prominent antibacterial ability and favorable osteogenic activity, revealing great application potential. Compared with conventional periodontal treatment, this local targeted drug delivery strategy effectively overcomes the shortcomings of frequent administration and systemic side effects. Consequently, the DOX-loaded composite nanoparticles possess excellent clinical value and developmental prospects for targeted periodontitis therapy.</p>

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Layer-by-layer self-assembled doxycycline/chitosan/silk fibroin nanoparticles for periodontitis

  • 期刊论文

  • 发表日期：

    2026年8月4日

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  • Wenxin Meng,
  • Ya Gao,
  • Feng Wei,
  • Xinyue Guan,
  • Yuhui Liu,
  • Yumeng Luo,
  • Guomin Wu

摘要

Periodontitis is a highly prevalent chronic oral inflammatory disease. It is featured by sustained inflammation, progressive periodontal tissue damage, and irreversible alveolar bone resorption, which remains a critical clinical challenge. In this study, a core–shell composite silk fibroin nanoparticle delivery system loaded with doxycycline (DOX) was successfully constructed. Firstly, ethanol-induced modification combined with silk fibroin self-assembly technology was applied to fabricate DOX-loaded silk fibroin microspheres. Based on electrostatic adsorption, layer-by-layer (LBL) self-assembly was further adopted to alternately adsorb chitosan (CS)-DOX and silk fibroin on the microsphere surface, thereby obtaining core–shell structured composite nanoparticles with a drug-loaded inner core. The as-prepared nanocarrier achieves favorable sustained drug release, antibacterial activity, and osteogenic potency. In vitro and in vivo experiments verified that the synthesized nanoparticles exhibit prominent antibacterial ability and favorable osteogenic activity, revealing great application potential. Compared with conventional periodontal treatment, this local targeted drug delivery strategy effectively overcomes the shortcomings of frequent administration and systemic side effects. Consequently, the DOX-loaded composite nanoparticles possess excellent clinical value and developmental prospects for targeted periodontitis therapy.