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<p>Semiconductor quantum dots (QDs) are widely used as promising biomarkers due to their high fluorescence quantum yield and stability. This study addresses CdSe/ZnS QDs that are bright and stable fluorescent materials with a hydrophilic surface coating providing biocompatibility and functional COOH groups for chemical reactions. QDs conjugated with aptamers open up new technologies for labeling biological systems. Therefore, this work is aimed at creating conjugates of CdSe/ZnS QDs with DNA aptamers (Gol1, U8, and U31) that combine the optical properties of nanoparticles and the specificity of aptamers to various targets. The aptamers used demonstrate specificity for the extracellular part of the epidermal growth factor receptor (EGFR), the overexpression of which is one of the key tumor markers in biomedical studies of glioblastoma. The paper proposes a method for creating QD and aptamer complexes by interacting carboxyl groups on the surface of nanoparticles and amino groups at the 5′-ends of aptamers. The formation of a conjugate due to an amide bond was demonstrated using IR spectroscopy. Attachment of aptamer molecules to QD leads to an increase in the average hydrodynamic radii of nanoparticles from 10.8 to 14.6–17.0&#xa0;nm. An ELISA-like assay based on the synthesized QD-aptamer conjugates showed selective interaction with the target protein (EGFR). The assay’s fluorescent results can be visualized upon excitation with UV light or recorded using a microplate reader. The signal is also observed at a concentration of QD-aptamer sols of 0.01&#xa0;µM, which makes this method sufficiently sensitive as an immunosorbent assay.</p> Graphical Abstract <p></p>

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Synthesis of CdSe/ZnS quantum dot-aptamer conjugates for epidermal growth factor receptor identification

  • 期刊论文

  • 发表日期：

    2025年10月26日

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  • Oleg N. Karpov,
  • Maria V. Grigoreva,
  • Olga A. Otmakhova,
  • Raisa V. Talroze

摘要

Semiconductor quantum dots (QDs) are widely used as promising biomarkers due to their high fluorescence quantum yield and stability. This study addresses CdSe/ZnS QDs that are bright and stable fluorescent materials with a hydrophilic surface coating providing biocompatibility and functional COOH groups for chemical reactions. QDs conjugated with aptamers open up new technologies for labeling biological systems. Therefore, this work is aimed at creating conjugates of CdSe/ZnS QDs with DNA aptamers (Gol1, U8, and U31) that combine the optical properties of nanoparticles and the specificity of aptamers to various targets. The aptamers used demonstrate specificity for the extracellular part of the epidermal growth factor receptor (EGFR), the overexpression of which is one of the key tumor markers in biomedical studies of glioblastoma. The paper proposes a method for creating QD and aptamer complexes by interacting carboxyl groups on the surface of nanoparticles and amino groups at the 5′-ends of aptamers. The formation of a conjugate due to an amide bond was demonstrated using IR spectroscopy. Attachment of aptamer molecules to QD leads to an increase in the average hydrodynamic radii of nanoparticles from 10.8 to 14.6–17.0 nm. An ELISA-like assay based on the synthesized QD-aptamer conjugates showed selective interaction with the target protein (EGFR). The assay’s fluorescent results can be visualized upon excitation with UV light or recorded using a microplate reader. The signal is also observed at a concentration of QD-aptamer sols of 0.01 µM, which makes this method sufficiently sensitive as an immunosorbent assay.

Graphical Abstract