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Background <p>Bioactive compounds are recognized for their ability to modulate immune cell activity and reduce inflammation. Polyphenols derived from Aronia leaves offer multiple biological activities; however, their role in regulating inflammatory responses remains poorly understood. This study aimed to evaluate the anti-inflammatory activity of Aronia melanocarpa leaf phenolic extract (APE) in LPS-stimulated RAW264.7 cells.</p> Methods and results <p>At concentrations up to 50&#xa0;µg/mL, APE exerted no cytotoxic effects in MTT assays performed with and without 1&#xa0;µg/mL LPS-stimulated macrophages. APE significantly inhibited nitric oxide production in LPS-stimulated cells, as determined by the Griess method. Furthermore, treatment with APE (10, 25, 50&#xa0;µg/mL) effectively decreased mRNA and protein expression of pro-inflammatory mediators, including IL-1β, COX-2 and iNOS, as analyzed by qPCR and Western blot, respectively. Additionally, Western blot analysis showed that APE modulated the NF-κB signaling pathway by downregulating the phosphorylation of NF-κB p65 and IκB-α, thereby suppressing NF-κB activation in LPS-stimulated RAW264.7 cells.</p> Conclusions <p>The findings suggest that polyphenols in Aronia leaves exert potent anti-inflammatory effects by inhibiting the NF-κB signaling pathway and suppressing pro-inflammatory mediators. Therefore, Aronia leaf extract may serve as a promising candidate for the development of therapeutic agents that support the treatment of inflammation-related diseases.</p>

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Anti-inflammatory potential of Aronia melanocarpa leaf phenolic extract via NF-κB pathway suppression in LPS-stimulated RAW264.7 macrophages

  • 期刊论文

  • 发表日期：

    2026年3月13日

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  • Katarzyna Owczarek,
  • Miłosz Caban,
  • Karolina Niewinna,
  • Dorota Sosnowska,
  • Dominika Kajszczak,
  • Urszula Lewandowska

摘要

Background

Bioactive compounds are recognized for their ability to modulate immune cell activity and reduce inflammation. Polyphenols derived from Aronia leaves offer multiple biological activities; however, their role in regulating inflammatory responses remains poorly understood. This study aimed to evaluate the anti-inflammatory activity of Aronia melanocarpa leaf phenolic extract (APE) in LPS-stimulated RAW264.7 cells.

Methods and results

At concentrations up to 50 µg/mL, APE exerted no cytotoxic effects in MTT assays performed with and without 1 µg/mL LPS-stimulated macrophages. APE significantly inhibited nitric oxide production in LPS-stimulated cells, as determined by the Griess method. Furthermore, treatment with APE (10, 25, 50 µg/mL) effectively decreased mRNA and protein expression of pro-inflammatory mediators, including IL-1β, COX-2 and iNOS, as analyzed by qPCR and Western blot, respectively. Additionally, Western blot analysis showed that APE modulated the NF-κB signaling pathway by downregulating the phosphorylation of NF-κB p65 and IκB-α, thereby suppressing NF-κB activation in LPS-stimulated RAW264.7 cells.

Conclusions

The findings suggest that polyphenols in Aronia leaves exert potent anti-inflammatory effects by inhibiting the NF-κB signaling pathway and suppressing pro-inflammatory mediators. Therefore, Aronia leaf extract may serve as a promising candidate for the development of therapeutic agents that support the treatment of inflammation-related diseases.