Scholarlink

Background <p>The genus <i>Streptomyces</i> are renowned for their ability to produce a wide array of antimicrobial compounds. However, their biosynthetic potential in polar environments, particularly the Southern Ocean, remains largely unexplored.</p> Methods and results <p>In this study, we isolated a <i>Streptomyces</i> strain SOSIST-3 from the Indian sector of the Southern Ocean. Using a combination of physiological assays and phylogenomic analysis, we identified the strain as <i>Streptomyces rochei</i>. The genome of this strain is 8.59&#xa0;Mb in size, with a GC content of 71%, and encodes 7,728 genes, including 590 core genes. Analysis of secondary metabolic potential using antiSMASH predicted 43 biosynthetic gene clusters, of which 19 showed low or no similarity to known clusters, indicating the presence of potentially novel biosynthetic pathways. Comparative genomic analysis with closely related <i>Streptomyces</i> using BiG-SCAPE revealed that strain SOSIST-3 harbours the most diverse BGC repertoire, encompassing nine different classes and a total of 21 biosynthetic genes. Antimicrobial activity assay demonstrated strong inhibitory effects against several pathogens. The highest inhibition was observed against <i>Aeromonas hydrophila</i> ATCC 7966 (23.8 ± 1.51&#xa0;mm), followed by <i>Klebsiella pneumoniae</i> ATCC 13,882 (18 ± 1.21&#xa0;mm), <i>Staphylococcus aureus</i> ATCC 29,213 (12.6 ± 1.19&#xa0;mm), <i>Pseudomonas aeruginosa</i> ATCC 27,853 (12.4 ± 1.12&#xa0;mm), and <i>Escherichia coli.</i> ATCC 25,922 (12.2 ± 1.04&#xa0;mm).</p> Conclusions <p>The findings highlight the untapped potential of <i>Streptomyces</i> from polar regions for antimicrobial discovery, with <i>S. rochei</i> SOSIST-3 emerging as a promising candidate for bioprospecting.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Comparative genomic and phenotypic analysis of Streptomyces rochei SOSIST-3 isolated from the Southern Ocean

  • 期刊论文

  • 发表日期：

    2026年1月16日

查看全文
  • Manigundan Kaari,
  • Jojy John,
  • Radhakrishnan Manikkam,
  • Amit Kumar,
  • Abirami Baskaran,
  • Parli Venkateswaran Bhaskar

摘要

Background

The genus Streptomyces are renowned for their ability to produce a wide array of antimicrobial compounds. However, their biosynthetic potential in polar environments, particularly the Southern Ocean, remains largely unexplored.

Methods and results

In this study, we isolated a Streptomyces strain SOSIST-3 from the Indian sector of the Southern Ocean. Using a combination of physiological assays and phylogenomic analysis, we identified the strain as Streptomyces rochei. The genome of this strain is 8.59 Mb in size, with a GC content of 71%, and encodes 7,728 genes, including 590 core genes. Analysis of secondary metabolic potential using antiSMASH predicted 43 biosynthetic gene clusters, of which 19 showed low or no similarity to known clusters, indicating the presence of potentially novel biosynthetic pathways. Comparative genomic analysis with closely related Streptomyces using BiG-SCAPE revealed that strain SOSIST-3 harbours the most diverse BGC repertoire, encompassing nine different classes and a total of 21 biosynthetic genes. Antimicrobial activity assay demonstrated strong inhibitory effects against several pathogens. The highest inhibition was observed against Aeromonas hydrophila ATCC 7966 (23.8 ± 1.51 mm), followed by Klebsiella pneumoniae ATCC 13,882 (18 ± 1.21 mm), Staphylococcus aureus ATCC 29,213 (12.6 ± 1.19 mm), Pseudomonas aeruginosa ATCC 27,853 (12.4 ± 1.12 mm), and Escherichia coli. ATCC 25,922 (12.2 ± 1.04 mm).

Conclusions

The findings highlight the untapped potential of Streptomyces from polar regions for antimicrobial discovery, with S. rochei SOSIST-3 emerging as a promising candidate for bioprospecting.