Scholarlink

Background <p>Rice cultivation in coastal and flood-prone regions is severely constrained by concurrent abiotic stresses such as salinity and submergence coupled with low P availability. Developing rice varieties with combined tolerance to these stresses is vital for ensuring sustainable productivity. The present study aimed to pyramid three key abiotic stress tolerance genes viz., <i>Pup1</i> (low P tolerance) and <i>Saltol1</i> (salinity tolerance) into the elite rice variety CR 1009 <i>Sub1</i> through marker-assisted backcross breeding (MABB).</p> Methods and results <p>An intercross was made between two backcross inbred lines, BIL 1094 (CR 1009 <i>Sub1</i> + <i>Saltol1</i>) and D5-1-3-2-1 (CR 1009 <i>Sub1</i> + <i>Pup1</i>), followed by two rounds of backcrossing and selfing to generate BC₂F₂ populations. Foreground selection using gene-specific SSR markers (K46-1 and K29-3 for <i>Pup1</i>, RM 3412 for <i>Saltol1</i> and ART 5 for <i>Sub1</i>) confirmed the presence of all three target loci. Background selection using 41 polymorphic SSR markers covering the whole chromosome revealed progressive genome recovery of the recurrent parent up to 94.8% in BC₂F₂. Residual heterozygosity and undesirable recombination events were minimal. Ten BC₂F₂ plants possessing all three target genes and high genomic similarity to CR 1009 <i>Sub1</i> were identified as promising lines.</p> Conclusions <p>This study highlights the effectiveness of MABB in combining multiple abiotic stress tolerance genes without compromising the agronomic traits of the recurrent. The selected lines hold significant potential for developing resilient rice cultivars suited to multi-stress environments, contributing to sustainable rice production in vulnerable agro-ecosystems.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Marker assisted pyramiding of Pup1 and Saltol1 QTLs and recurrent parent genome recovery in elite rice variety CR 1009 Sub1

  • 期刊论文

  • 发表日期：

    2026年1月14日

查看全文
  • Raiza Christina G.,
  • Aananthi N.,
  • Gunasekaran M.,
  • Gnanamalar R.P.,
  • Merina Prem Kumari S.,
  • Amutha R.,
  • Saravana Pandian P.

摘要

Background

Rice cultivation in coastal and flood-prone regions is severely constrained by concurrent abiotic stresses such as salinity and submergence coupled with low P availability. Developing rice varieties with combined tolerance to these stresses is vital for ensuring sustainable productivity. The present study aimed to pyramid three key abiotic stress tolerance genes viz., Pup1 (low P tolerance) and Saltol1 (salinity tolerance) into the elite rice variety CR 1009 Sub1 through marker-assisted backcross breeding (MABB).

Methods and results

An intercross was made between two backcross inbred lines, BIL 1094 (CR 1009 Sub1 + Saltol1) and D5-1-3-2-1 (CR 1009 Sub1 + Pup1), followed by two rounds of backcrossing and selfing to generate BC₂F₂ populations. Foreground selection using gene-specific SSR markers (K46-1 and K29-3 for Pup1, RM 3412 for Saltol1 and ART 5 for Sub1) confirmed the presence of all three target loci. Background selection using 41 polymorphic SSR markers covering the whole chromosome revealed progressive genome recovery of the recurrent parent up to 94.8% in BC₂F₂. Residual heterozygosity and undesirable recombination events were minimal. Ten BC₂F₂ plants possessing all three target genes and high genomic similarity to CR 1009 Sub1 were identified as promising lines.

Conclusions

This study highlights the effectiveness of MABB in combining multiple abiotic stress tolerance genes without compromising the agronomic traits of the recurrent. The selected lines hold significant potential for developing resilient rice cultivars suited to multi-stress environments, contributing to sustainable rice production in vulnerable agro-ecosystems.