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<p>Black gram [Vigna mungo (L.) Hepper] is an important food legume whose productivity in the Indian subcontinent has declined due to multiple abiotic and biotic stresses, particularly Yellow Mosaic Virus (YMV), a major yield-limiting pathogen. To support the development of YMV-resistant varieties, the present study aimed to identify reliable molecular markers associated with YMV resistance. A bi-parental mapping population was developed from two contrasting genotypes—NUK 17 − 05 (highly resistant) and VUG 49 (highly susceptible)—and subjected to bulked segregant analysis using RAPD markers. A polymorphic RAPD marker (OPAD17) linked to resistance was identified and subsequently converted into a stable SCAR marker, designated SCAR15 (238&#xa0;bp). SCAR15 consistently differentiated resistant and susceptible parents based on band intensity and was validated using F₂ individuals representing extreme phenotypes. qRT-PCR analysis confirmed that resistance was associated with copy number variation at the SCAR15 locus. Resistant lines showed a copy number &gt; 0.1 with a mean value of 1.85, whereas susceptible lines had a mean copy number of 0.06. These results demonstrate that YMV resistance in the studied population is linked to genomic copy number variation. The SCAR15 marker developed in this study provides a robust tool for marker-assisted selection and breeding of YMV-resistant black gram genotypes.</p>

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Development of SCAR and rapid detection of YMV resistant genotypes of blackgram through qRT-PCR based absolute quantification

  • 期刊论文

  • 发表日期：

    2026年1月6日

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  • Priyanka Halldakeri,
  • Amar A. Sakure,
  • Sushil Kumar,
  • Sneha Macwana,
  • K. V. Patel,
  • D. A. Patel

摘要

Black gram [Vigna mungo (L.) Hepper] is an important food legume whose productivity in the Indian subcontinent has declined due to multiple abiotic and biotic stresses, particularly Yellow Mosaic Virus (YMV), a major yield-limiting pathogen. To support the development of YMV-resistant varieties, the present study aimed to identify reliable molecular markers associated with YMV resistance. A bi-parental mapping population was developed from two contrasting genotypes—NUK 17 − 05 (highly resistant) and VUG 49 (highly susceptible)—and subjected to bulked segregant analysis using RAPD markers. A polymorphic RAPD marker (OPAD17) linked to resistance was identified and subsequently converted into a stable SCAR marker, designated SCAR15 (238 bp). SCAR15 consistently differentiated resistant and susceptible parents based on band intensity and was validated using F₂ individuals representing extreme phenotypes. qRT-PCR analysis confirmed that resistance was associated with copy number variation at the SCAR15 locus. Resistant lines showed a copy number > 0.1 with a mean value of 1.85, whereas susceptible lines had a mean copy number of 0.06. These results demonstrate that YMV resistance in the studied population is linked to genomic copy number variation. The SCAR15 marker developed in this study provides a robust tool for marker-assisted selection and breeding of YMV-resistant black gram genotypes.