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Background <p>Banana streak MY virus (BSMYV) [<i>Badnavirus gammavirgamusae</i>] is one of the most prevalent badnaviruses associated with streak disease in <i>Musa</i> species. Its infection and symptom development vary across the banana genotypes. Present study, for the first time attempted successful agroinfection of BSMYV in diploid banana progenitor <i>Musa balbisiana</i> cv. Bhimkol (BB) which was earlier considered resistant and elucidated the underlying mechanism of infection.</p> Methods and results <p>Agroinfection of Bhimkol banana showed delayed infection at 280 days post-BSMYV agroinoculation. High-throughput sequencing (HTS) was carried out to decipher the mechanisms of delayed BSMYV infection. <i>De novo</i> assembly of transcriptome generated 101,961 unigenes with a mean length of 1310 base pairs (bp). Out of the total coding sequence (CDS), 48,797 were annotated against the non-redundant protein database. A total of 3174 differentially expressed genes (DEGs) were identified, of which 1138 up-regulated and 2036 down-regulated genes were observed in comparison of mock inoculated (BB_Mock) and delayed infected (BB_Symptomatic) Bhimkol banana. A heatmap with hierarchical clustering of top 50 significantly differentially expressed genes showed four different clusters. Based on fold changes, and adjusted p-value, twelve selected DEGs were validated using quantitative-PCR (qPCR). Selected DEGs were associated with regulation of plant hormone, host defense system and host machinery hijack. Enrichment analysis of differentially expressed genes hints toward the importance of Ca<sup>2+</sup> dependent Ras signaling in <i>M. balbisiana</i> (BB) exhibiting delayed BSMYV infection response.</p> Conclusion <p>The present study revealed a complex cross-talk between Ca<sup>2+</sup> dependent Ras, macromolecules localization and auxin responsive genes to regulate primary metabolite production that possibly hinder the spread of virus across the cell, leading to the delayed establishment of BSMYV infection in Bhimkol banana. To the best of our knowledge, this is first report on elucidation of delayed infection of BSMYV in diploid <i>M. balbisiana</i> (BB) through comprehensive transcriptome analysis.</p>

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Molecular basis of delayed BSMYV infection in diploid Musa balbisiana, a banana progenitor

  • 期刊论文

  • 发表日期：

    2025年12月10日

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  • Susheel Kumar Sharma,
  • Albert Maibam,
  • Richa Rai,
  • Neeta Pathaw,
  • Damini Diksha,
  • Sapam Monteshori,
  • Yumlembam Rupert Anand,
  • Subhra Saikat Roy,
  • Akoijam Ratankumar Singh,
  • Nitika Gupta,
  • Virendra Kumar Baranwal

摘要

Background

Banana streak MY virus (BSMYV) [Badnavirus gammavirgamusae] is one of the most prevalent badnaviruses associated with streak disease in Musa species. Its infection and symptom development vary across the banana genotypes. Present study, for the first time attempted successful agroinfection of BSMYV in diploid banana progenitor Musa balbisiana cv. Bhimkol (BB) which was earlier considered resistant and elucidated the underlying mechanism of infection.

Methods and results

Agroinfection of Bhimkol banana showed delayed infection at 280 days post-BSMYV agroinoculation. High-throughput sequencing (HTS) was carried out to decipher the mechanisms of delayed BSMYV infection. De novo assembly of transcriptome generated 101,961 unigenes with a mean length of 1310 base pairs (bp). Out of the total coding sequence (CDS), 48,797 were annotated against the non-redundant protein database. A total of 3174 differentially expressed genes (DEGs) were identified, of which 1138 up-regulated and 2036 down-regulated genes were observed in comparison of mock inoculated (BB_Mock) and delayed infected (BB_Symptomatic) Bhimkol banana. A heatmap with hierarchical clustering of top 50 significantly differentially expressed genes showed four different clusters. Based on fold changes, and adjusted p-value, twelve selected DEGs were validated using quantitative-PCR (qPCR). Selected DEGs were associated with regulation of plant hormone, host defense system and host machinery hijack. Enrichment analysis of differentially expressed genes hints toward the importance of Ca2+ dependent Ras signaling in M. balbisiana (BB) exhibiting delayed BSMYV infection response.

Conclusion

The present study revealed a complex cross-talk between Ca2+ dependent Ras, macromolecules localization and auxin responsive genes to regulate primary metabolite production that possibly hinder the spread of virus across the cell, leading to the delayed establishment of BSMYV infection in Bhimkol banana. To the best of our knowledge, this is first report on elucidation of delayed infection of BSMYV in diploid M. balbisiana (BB) through comprehensive transcriptome analysis.