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Background <p>Zearalenone (ZEN), an important mycotoxin produced by <i>Fusarium</i> species, poses significant health threat due to estrogenic and cytotoxic effects. This study aimed to investigate protective effects of <i>Lactobacillus acidophilus</i> (<i>L. acidophilus</i>) against on ZEN-induced cytotoxicity and its influence on miRNA-34a expression in HepG2 cell line.</p> Methods <p>HepG2 cells were seeded in 96-well plates and incubated at 37&#xa0;°C for 24&#xa0;h; then survival rate of HepG2 cells treated with different concentrations of 100–200µM zearalenone and probiotics was calculated using MTT method and IC50 value was calculated using Prism version 9 software. Lactate dehydrogenase activity, a marker of cellular damage, was measured. Additionally, the expression of miRNA-34a, a microRNA potentially involved in ZEN-mediated cytotoxicity, was quantified using real-time PCR with specific primers.</p> Results <p>The expression of miRNA-34a gene in HepG2 cells exposed to ZEN treated with <i>L. acidophilus</i>.</p> Conclusion <p>The results of the present study showed probiotics moderate the toxic effects of this mycotoxin.</p>

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The role of miR-34a gene expression in Zearalenone-mediated toxicity in vitro: the protective effect of Lactobacillus acidophilus

  • 期刊论文

  • 发表日期：

    2025年11月28日

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  • Sodabe Khodabandehlou,
  • Fatemeh Nouri,
  • Mohammad Reza Arabestani,
  • Ali Heshmati,
  • Akram Ranjbar

摘要

Background

Zearalenone (ZEN), an important mycotoxin produced by Fusarium species, poses significant health threat due to estrogenic and cytotoxic effects. This study aimed to investigate protective effects of Lactobacillus acidophilus (L. acidophilus) against on ZEN-induced cytotoxicity and its influence on miRNA-34a expression in HepG2 cell line.

Methods

HepG2 cells were seeded in 96-well plates and incubated at 37 °C for 24 h; then survival rate of HepG2 cells treated with different concentrations of 100–200µM zearalenone and probiotics was calculated using MTT method and IC50 value was calculated using Prism version 9 software. Lactate dehydrogenase activity, a marker of cellular damage, was measured. Additionally, the expression of miRNA-34a, a microRNA potentially involved in ZEN-mediated cytotoxicity, was quantified using real-time PCR with specific primers.

Results

The expression of miRNA-34a gene in HepG2 cells exposed to ZEN treated with L. acidophilus.

Conclusion

The results of the present study showed probiotics moderate the toxic effects of this mycotoxin.